Detailed information of TA system TA191931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1852271..1853081
• Replicon: chromosome
• Accession: NZ_CP069861
• Organism: Klebsiella pneumoniae strain FDAARGOS_1311

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A332NV98
• Locus tag: I6K41_RS08920
• Protein ID: WP_014908042.1
• Coordinates: 1852548..1853081 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: I6K41_RS08915
• Protein ID: WP_023343051.1
• Coordinates: 1852271..1852537 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K41_RS08895	1848142..1848486	-	345	WP_004192220.1	cation efflux system protein CusF	-
I6K41_RS08900	1848504..1849889	-	1386	WP_024623207.1	efflux transporter outer membrane subunit	-
I6K41_RS08905	1850062..1850745	+	684	WP_002887273.1	copper response regulator transcription factor CusR	-
I6K41_RS08910	1850735..1852168	+	1434	WP_204745768.1	Cu(+)/Ag(+) sensor histidine kinase	-
I6K41_RS08915	1852271..1852537	+	267	WP_023343051.1	DUF1778 domain-containing protein	Antitoxin
I6K41_RS08920	1852548..1853081	+	534	WP_014908042.1	GNAT family N-acetyltransferase	Toxin
I6K41_RS08925	1853129..1854250	-	1122	WP_012737335.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19795.68 Da
• Isoelectric Point: 5.2614

>T191931 WP_014908042.1 NZ_CP069861:1852548-1853081 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T191931 NZ_CP091998:4457589-4457900 [Salmonella enterica subsp. enterica serovar Kentucky]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10021.46 Da
• Isoelectric Point: 10.5807

>AT191931 WP_023343051.1 NZ_CP069861:1852271-1852537 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRRVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT191931 NZ_CP091998:4457897-4458343 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: A0A332NV98