191928

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1230424..1231049 Replicon chromosome
Accession NZ_CP069861
Organism Klebsiella pneumoniae strain FDAARGOS_1311

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I6K41_RS06100 Protein ID WP_002882817.1
Coordinates 1230666..1231049 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I6K41_RS06095 Protein ID WP_004150355.1
Coordinates 1230424..1230666 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K41_RS06070 1226415..1227014 + 600 WP_004151865.1 glucose-1-phosphatase -
I6K41_RS06075 1227008..1227868 + 861 WP_004146232.1 virulence factor BrkB family protein -
I6K41_RS06080 1227865..1228302 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I6K41_RS06085 1228347..1229288 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I6K41_RS06090 1229302..1230219 - 918 WP_023302328.1 alpha/beta hydrolase -
I6K41_RS06095 1230424..1230666 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I6K41_RS06100 1230666..1231049 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I6K41_RS06105 1231223..1232152 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I6K41_RS06110 1232149..1232784 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I6K41_RS06115 1232781..1233683 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T191928 WP_002882817.1 NZ_CP069861:1230666-1231049 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T191928 NZ_CP091998:3457557-3457775 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT191928 WP_004150355.1 NZ_CP069861:1230424-1230666 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT191928 NZ_CP091998:3457155-3457529 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References