Detailed information of TA system TA191903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2560..3148
• Replicon: plasmid unnamed
• Accession: NZ_CP069852
• Organism: Acinetobacter baumannii strain FDAARGOS_1359

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A3G6Z3M6
• Locus tag: I6K89_RS18485
• Protein ID: WP_000438826.1
• Coordinates: 2861..3148 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9M5I3
• Locus tag: I6K89_RS18480
• Protein ID: WP_001983304.1
• Coordinates: 2560..2874 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K89_RS18475	21..2432	-	2412	WP_000932953.1	TonB-dependent receptor ZnuD2	-
I6K89_RS18480	2560..2874	-	315	WP_001983304.1	BrnA antitoxin family protein	Antitoxin
I6K89_RS18485	2861..3148	-	288	WP_000438826.1	BrnT family toxin	Toxin
I6K89_RS18490	3521..4039	+	519	WP_000447193.1	sel1 repeat family protein	-
I6K89_RS18495	4228..4371	-	144	WP_001125246.1	hypothetical protein	-
I6K89_RS18500	4391..4966	-	576	WP_001096616.1	helix-turn-helix domain-containing protein	-
I6K89_RS18505	4959..5909	-	951	WP_001205343.1	replication initiation protein RepM	-
I6K89_RS18510	7119..7490	+	372	WP_000504218.1	hypothetical protein	-
I6K89_RS18515	7490..7663	+	174	WP_001282484.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..8731	8731

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11303.72 Da
• Isoelectric Point: 6.0889

>T191903 WP_000438826.1 NZ_CP069852:c3148-2861 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T191903 NZ_CP091992:c2752082-2751980 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12208.84 Da
• Isoelectric Point: 7.2026

>AT191903 WP_001983304.1 NZ_CP069852:c2874-2560 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT191903 NZ_CP091992:2751973-2752118 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A3G6Z3M6

Antitoxin

• AlphaFold DB: N9M5I3