Detailed information of TA system TA191899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4108842..4109617
• Replicon: chromosome
• Accession: NZ_CP069847
• Organism: Klebsiella pneumoniae strain FDAARGOS_1305

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: I6K35_RS22075
• Protein ID: WP_057222542.1
• Coordinates: 4109132..4109617 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: W8UEW1
• Locus tag: I6K35_RS22070
• Protein ID: WP_004150912.1
• Coordinates: 4108842..4109135 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K35_RS22050	4104050..4104652	-	603	WP_004174410.1	short chain dehydrogenase	-
I6K35_RS22055	4104750..4105661	+	912	WP_204752498.1	LysR family transcriptional regulator	-
I6K35_RS22060	4105662..4106810	-	1149	WP_016947441.1	PLP-dependent aspartate aminotransferase family protein	-
I6K35_RS22065	4106821..4108197	-	1377	WP_072143321.1	cystathionine beta-synthase	-
I6K35_RS22070	4108842..4109135	+	294	WP_004150912.1	DUF1778 domain-containing protein	Antitoxin
I6K35_RS22075	4109132..4109617	+	486	WP_057222542.1	GNAT family N-acetyltransferase	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-95)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17482.40 Da
• Isoelectric Point: 7.7532

>T191899 WP_057222542.1 NZ_CP069847:4109132-4109617 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGNGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLGDLVN
A

Nucleotide

• Length: 486 bp

>T191899 NZ_CP091986:273317-273601 [Klebsiella pneumoniae]
ATGACCTATAAGTTAGCCTTCAATGAATCAGCTTTGAAAGAATGGAAAAAACTGGGTCATACTATTCGGGAGCAATTCAA
AAAGAAGCTTTCCGAACGGCTTCAGAACCCACGGGTGCCCGCAGCACAACTCCATGGAGGTAAAGACCAGTATAAAATAA
AGCTCCATGGCGCGGGTTACCGACTGGTTTACAGTGTCAACGATGAAATTGTGACGGTCACCGTAATTGGGGTGGGCAAA
CGCAACAATGATGATATCTACAACGCAACCCGACACAGAAATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10901.46 Da
• Isoelectric Point: 6.3783

>AT191899 WP_004150912.1 NZ_CP069847:4108842-4109135 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Nucleotide

• Length: 294 bp

>AT191899 NZ_CP091986:273079-273327 [Klebsiella pneumoniae]
ATGGCATTTCAGGTTTTAACCACCACAGCAGCCAGCATTACAGAACTTAAACGTGACCCTATGGGTACCTTCAATGCAGG
AGAAGGTGCACCGGTAGCAATCCTGAATCGCAACGAACCCGCTTTTTACTGTGTACCACCAGCCCTGTATGCTCAGCTGA
TGGAAATGCTGGAAGATCAAGAGTTGGGGCGTATTGCTGATGAACGAATCGGTGAGGACCGAATCGAGGTCAACATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GVL4