Detailed information of TA system TA191898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 3717317..3717903
• Replicon: chromosome
• Accession: NZ_CP069847
• Organism: Klebsiella pneumoniae strain FDAARGOS_1305

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: I6K35_RS20040
• Protein ID: WP_002920800.1
• Coordinates: 3717535..3717903 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: I6K35_RS20035
• Protein ID: WP_004174006.1
• Coordinates: 3717317..3717538 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K35_RS20015	3713474..3714400	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
I6K35_RS20020	3714397..3715674	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
I6K35_RS20025	3715671..3716438	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
I6K35_RS20030	3716440..3717153	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
I6K35_RS20035	3717317..3717538	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I6K35_RS20040	3717535..3717903	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
I6K35_RS20045	3718176..3719492	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
I6K35_RS20050	3719599..3720486	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
I6K35_RS20055	3720483..3721328	+	846	WP_004174009.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
I6K35_RS20060	3721330..3722400	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3712303..3723137	10834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T191898 WP_002920800.1 NZ_CP069847:3717535-3717903 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T191898 NZ_CP091985:c742858-742457 [Burkholderia vietnamiensis]
ATGCCGCGTTACATGCTCGACACCAACATGTGCATCTACTTGATGAAGAGCCAGCCCGAGCAAGTGGCGAAGCGATTTGC
GCAGTGCTACACGGGCGACGTCGTGATGTCGGCGATCACGTATGCCGAGCTGGAGTACGGCGTCACCGCTTGCGCGGCGC
CCGCGCGTGAGCGCCGCCATCTTGCGGCGCTGATCGAGGATATTCCCGTCGCGCCGTTCGACGCCGCGGCCGCGCAAGCA
TATGGGCCCGTGCGTGACGCAACGCGCGCATGGAAGAAGGATCATCTCGACAAACTGATCGCCGCTCATGCGGTGTCGCT
CGATGTCGTGCCGGTGACGAATGACGAGCGCGATTTCACGAGCTATCCGGGATTGCGGCTGGAGAACTGGCTGAACGATT
AG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT191898 WP_004174006.1 NZ_CP069847:3717317-3717538 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT191898 NZ_CP091985:c743094-742858 [Burkholderia vietnamiensis]
ATGCATACCACGAGGGTATTTCGGAACGGCAATTCGCAGGCCGTGCGAATTCCTGCGGATCTCGCTTATGAGCGCAGCGA
TATCGAGCTCGAGATCGAGCGCATCGGCGACGAGATCCGCATCCGGCCGGCGCGACGGCCGTTGACGCAGGTGCTCAAGA
AGTTCGCGAAGTTCGCGCCCGATTTCATGGCGGAAGGGCGTGGCGACCACGAGCAGGCCGAACGCGAGGGGTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7