Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3316483..3317108 Replicon chromosome
Accession NZ_CP069847
Organism Klebsiella pneumoniae strain FDAARGOS_1305

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I6K35_RS18180 Protein ID WP_002882817.1
Coordinates 3316483..3316866 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I6K35_RS18185 Protein ID WP_004150355.1
Coordinates 3316866..3317108 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K35_RS18165 3313849..3314751 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
I6K35_RS18170 3314748..3315383 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I6K35_RS18175 3315380..3316309 + 930 WP_004146237.1 formate dehydrogenase accessory protein FdhE -
I6K35_RS18180 3316483..3316866 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I6K35_RS18185 3316866..3317108 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I6K35_RS18190 3317313..3318230 + 918 WP_004895173.1 alpha/beta hydrolase -
I6K35_RS18195 3318244..3319185 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I6K35_RS18200 3319230..3319667 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I6K35_RS18205 3319664..3320524 - 861 WP_023280688.1 virulence factor BrkB family protein -
I6K35_RS18210 3320518..3321117 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T191897 WP_002882817.1 NZ_CP069847:c3316866-3316483 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T191897 NZ_CP091985:c108674-108255 [Burkholderia vietnamiensis]
ATGTTTTTGGTTGATACCAACGTCATCAGCGAAGTACGGAAGCGGGAGCGAGCAGATAAAGGTGTGATGGCGTTTTTCCG
CAAGGCCGCGCAGGACGATGCCGATCTGTACCTTTCCGTGGTGACGGTTGGCGAACTGCGGCGCGGTGTGGAAATCATCC
GTCACCGTGGCGACAAGTCCCAGGCGACGCGCCTTGAAAACTGGCTCGATGGCGTACTTCGGGAGTTTGCGTCGAACATT
CTGGCGGTGGACGAAGAGATCGGGCAGTTGTGGGGACGCCTGCGCGTCCCGCATCCCGAGCACTCGCTCGACAAGTTGAT
CGCGGCAACGGCGTTGATCCACGACCTGATCGTCGTGACGCGGAACGTCGATGATTTCGCCGGAACGGGCGCACGCGTTC
TGAACCCATTCGAAAGCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT191897 WP_004150355.1 NZ_CP069847:c3317108-3316866 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT191897 NZ_CP091985:c108894-108661 [Burkholderia vietnamiensis]
ATGGCAACTCTTCTGGTGAGAAATGTGGACGAGGAACTCGTCCAAAGCCTGCGCGAGCGGGCCGCAGCCAACGGGCGTAG
TGCGGAGGCCGAGCATCGAGAGATCCTGGCAAAGGCGCTGCGCGCGCCGCAGCGCAAGACGTTCGCGCAGGTGTTGATGA
GTATCCCGAACGTCGGAACCGATGCCGATTTTGCGCGCGTGGATGACGGCGAGGCAGCGAATGTTTTTGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References