Detailed information of TA system TA191875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2913..3501
• Replicon: plasmid unnamed2
• Accession: NZ_CP069842
• Organism: Acinetobacter baumannii strain FDAARGOS_1360

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A0J8TIH5
• Locus tag: I6K90_RS19635
• Protein ID: WP_000438827.1
• Coordinates: 2913..3200 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9M5I3
• Locus tag: I6K90_RS19640
• Protein ID: WP_001983304.1
• Coordinates: 3187..3501 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K90_RS19615	453..1403	+	951	WP_001205343.1	replication initiation protein RepM	-
I6K90_RS19620	1396..1971	+	576	WP_001096616.1	helix-turn-helix domain-containing protein	-
I6K90_RS19625	1991..2134	+	144	WP_001125244.1	hypothetical protein	-
I6K90_RS19630	2319..2522	-	204	WP_000482656.1	hypothetical protein	-
I6K90_RS19635	2913..3200	+	288	WP_000438827.1	BrnT family toxin	Toxin
I6K90_RS19640	3187..3501	+	315	WP_001983304.1	BrnA antitoxin family protein	Antitoxin
I6K90_RS19645	3629..6040	+	2412	WP_000932953.1	TonB-dependent receptor ZnuD2	-
I6K90_RS19650	6345..6806	+	462	WP_000761346.1	DIP1984 family protein	-
I6K90_RS19655	7163..8328	-	1166	Protein_8	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..14879	14879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11329.75 Da
• Isoelectric Point: 5.6762

>T191875 WP_000438827.1 NZ_CP069842:2913-3200 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRYTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T191875 NZ_CP091977:2877794-2878204 [Aromatoleum aromaticum]
ATGTCGGTCCGCTATTTGCTCGACACGAACATCTGCATCTACATCGCGAAGCACAACCCGCCGGAAGTTCGCCAGCGCTT
CGCCCGCCACGCTGCATCCGAACTCGCGATGTCCGTCATCACGCTCGGCGAACTGCGCTTCGGCGCGGAGAAGAGCCAGG
CCCGCGACAAGGCGCTCGCTGCGATTCAAAGGCTGGAAGCGACGGTCCGGATCGCGCCACTGACGGAGTCGGTCGGCGAG
CACTACGGCCAGATCCGCGCGAATCTCCAGCACAAGGGCCAACCGATCGGCAACAACGACCTGTGGCTGGCCGCCCACGC
GCGCGCCGAAGGTTGGATCCTCGTGACAAACAACGAACGCGAATTCGTCCGCGTAGAAGGACTGCAAGTCGAAAACTGGG
TAGACCGATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12208.84 Da
• Isoelectric Point: 7.2026

>AT191875 WP_001983304.1 NZ_CP069842:3187-3501 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT191875 NZ_CP091977:2877563-2877793 [Aromatoleum aromaticum]
ATGGTTTACGCCCGCGTCTTCCGCTCCGGCAACAGCCAGGCGGTCCGCCTGCCGAAGGAGTTTCGGCTCGATACGGACCG
GGTCGAAATCTTCCGCCGCGGCGATGAAATCGTGCTGCGCGAACTGCCCGGCAATGCGGCCGCGATTTTCGATGCGCTAA
TCGAGCTGCCCGTCAACTTCATGTCTGAAGGGCGCAACGATGTCGCGCCACAGGAGCGGGAGGCGCTCTGA

Structures

Toxin

• AlphaFold DB: A0A0J8TIH5

Antitoxin

• AlphaFold DB: N9M5I3