Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 3953015..3953658 Replicon chromosome
Accession NZ_CP069821
Organism Klebsiella pneumoniae strain FDAARGOS_1326

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag I6K56_RS19135 Protein ID WP_032433387.1
Coordinates 3953015..3953431 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag I6K56_RS19140 Protein ID WP_001261276.1
Coordinates 3953428..3953658 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K56_RS19110 3948480..3948728 - 249 WP_032433382.1 hypothetical protein -
I6K56_RS19115 3949047..3949328 + 282 Protein_3734 AAA family ATPase -
I6K56_RS19120 3949394..3950362 - 969 WP_076026135.1 IS5-like element IS903B family transposase -
I6K56_RS19125 3950404..3951684 + 1281 Protein_3736 ATP-binding protein -
I6K56_RS19130 3951879..3952859 - 981 WP_032433385.1 hypothetical protein -
I6K56_RS19135 3953015..3953431 - 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
I6K56_RS19140 3953428..3953658 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
I6K56_RS19145 3953963..3954778 + 816 WP_032433388.1 hypothetical protein -
I6K56_RS19150 3955306..3957546 - 2241 WP_045326788.1 NTPase -
I6K56_RS19155 3957549..3958636 - 1088 Protein_3742 transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3924222..3969022 44800
- flank IS/Tn - - 3949394..3950317 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T191868 WP_032433387.1 NZ_CP069821:c3953431-3953015 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T191868 NZ_CP091977:2145013-2145384 [Aromatoleum aromaticum]
ATGCCATTCGTCCTGGATGTTAGCAAAATCGCTAACATCCAGGACATGAACTGGACAGTCAGTTACTTCAACGAGCGCGT
GCGGGAGGAAATCACCAAATGGCCAGTCGGCATCTACGCTGACTTCCTGCGCCTCATCTTCCTGATGGAAGAGCACGGTG
CTGACCTGCGCCTGCCGCACTCTCGCGCCATGGGCGATGGCCTCTTCGAGCTGCGCTGCAAGGGTGAGGAAGGAATTGGC
CGAGTGTTCTACTGCACGTTGGTCGGACGCCAGATCGTCATCCTGCACAGCTTCATCAAAAAGACGCAGGAAACTCCAGA
CCGGGAGCTGAAGACTGCCCGCAAGCGACTGAAGGAGGTCAAACATGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT191868 WP_001261276.1 NZ_CP069821:c3953658-3953428 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT191868 NZ_CP091977:2145377-2145688 [Aromatoleum aromaticum]
ATGGATAAAGAGCATTTCAAGCCGGTTCGCCTGGACACCAAGGCCGAACTTGCCCGTGCCATGCAACGGCCGGGATTCAA
GGCTGCGTGGGACGGACTGGAGGAAGAGTACGCGGCACTCAACGAGCTGCTCCAGGCGCGCAAGCAAGCCGGCCTGACGC
AGGAAGAAGTCGCTGCGCGCATGGGCACGACCAAGAGCGCCGTGTCGCGTCTGGAAGCATCCATTCGTAGCGAAAGCCAT
TCGCCCTCGTTCGCCACGCTGCGCAAGTACGCACACGCCTGCGGCAAGCGACTGGTCATCAAGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References