Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3935165..3935901 Replicon chromosome
Accession NZ_CP069821
Organism Klebsiella pneumoniae strain FDAARGOS_1326

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag I6K56_RS19030 Protein ID WP_032433360.1
Coordinates 3935165..3935647 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag I6K56_RS19035 Protein ID WP_003026799.1
Coordinates 3935635..3935901 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K56_RS19010 3931714..3932454 + 741 WP_050597964.1 molecular chaperone -
I6K56_RS19015 3932458..3933036 + 579 WP_032432061.1 type 1 fimbrial protein -
I6K56_RS19020 3933048..3933992 + 945 WP_077254249.1 fimbrial protein -
I6K56_RS19025 3934205..3934804 + 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
I6K56_RS19030 3935165..3935647 - 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
I6K56_RS19035 3935635..3935901 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
I6K56_RS19040 3936214..3936777 - 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
I6K56_RS19045 3936787..3937419 - 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
I6K56_RS19050 3937437..3938441 - 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
I6K56_RS19055 3938425..3939087 - 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
I6K56_RS19060 3939116..3940255 - 1140 WP_032433368.1 mannitol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3924222..3969022 44800


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T191867 WP_032433360.1 NZ_CP069821:c3935647-3935165 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T191867 NZ_CP091977:2095286-2095588 [Aromatoleum aromaticum]
ATGAGCAAACCAGTGAAAATCAGCGACCTGCCCGAGTTCGACATGGCAGAGCACCTGAAGACCGATGCCGATGTGGCGGA
ATACCTGCGCCAGGTACTCGAAGACGGCGAGCCCGGCGAACTTGCGGCTGCGCTCGGTCACATCGCCAAAGCGCGCGGCA
TGACCGAAATCGCCAAGGCCACCGGCATCAAGCGCGAAGCGCTTTACAAGGCGCTTCGGCCGAATGCCCACCCGCGCCTC
GAAACCGTTCAGCGCGTCTGCAAAGCGCTGGGGGTCAAGCTCACGGCGACGGTCGACAACTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT191867 WP_003026799.1 NZ_CP069821:c3935901-3935635 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT191867 NZ_CP091977:2094983-2095282 [Aromatoleum aromaticum]
ATGCCCAGCGTAAAACACACGCCTGAATTCGACGGCTGGCTCAACAGCTTACGGGACCGCCCCACGCGCTTCCGGCTGCT
GCGCCGGCTCGAAAAGGCGCAGCGCGGACTGTTGGGCGACGTTGCTCCAGTTGGCGAAGGTGTGTTCGAGATGCGCGAGT
TTTTTGGTCCCGGCTGGCGCATGTATTACCTCCAGCGCGGCGAAGTGCTGATCGTGATGCTGGGCGGCGGTGACAAGAGC
AGCCAGGCAGCGGACATCGCCTCCGCCAAGGCCTTGGCGGCGACGTTAAAGGACGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References