Detailed information of TA system TA191850

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4673848..4674554
• Replicon: chromosome
• Accession: NZ_CP069815
• Organism: Raoultella planticola strain HH15

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A443VFJ3
• Locus tag: G9395_RS22155
• Protein ID: WP_064793341.1
• Coordinates: 4674186..4674554 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A443VFG7
• Locus tag: G9395_RS22150
• Protein ID: WP_064793340.1
• Coordinates: 4673848..4674165 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9395_RS22110	4669434..4670144	+	711	WP_064793335.1	DeoR family transcriptional regulator	-
G9395_RS22115	4670170..4670274	+	105	Protein_4345	DUF4339 domain-containing protein	-
G9395_RS22120	4670435..4671490	+	1056	WP_064793336.1	hypothetical protein	-
G9395_RS22125	4671696..4671854	+	159	WP_081277336.1	DUF905 family protein	-
G9395_RS22130	4672069..4672527	+	459	WP_000526113.1	IS200/IS605-like element IS200C family transposase	-
G9395_RS22135	4672651..4673109	+	459	WP_064793337.1	antirestriction protein	-
G9395_RS22140	4673118..4673600	+	483	WP_165634230.1	DNA repair protein RadC	-
G9395_RS22145	4673609..4673830	+	222	WP_064793339.1	DUF987 domain-containing protein	-
G9395_RS22150	4673848..4674165	+	318	WP_064793340.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G9395_RS22155	4674186..4674554	+	369	WP_064793341.1	TA system toxin CbtA family protein	Toxin
G9395_RS22160	4674975..4675435	-	461	Protein_4354	transposase family protein	-
G9395_RS22165	4676040..4677089	+	1050	WP_064793343.1	LacI family transcriptional regulator	-
G9395_RS22170	4677150..4677569	-	420	WP_032689350.1	RbsD/FucU family protein	-
G9395_RS22175	4677615..4678379	-	765	WP_049266034.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	4672069..4675432	3363

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13683.99 Da
• Isoelectric Point: 8.7174

>T191850 WP_064793341.1 NZ_CP069815:4674186-4674554 [Raoultella planticola]
MKTLPAIISRAAKPCLSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTATDILQARKACGLMSRCSYREVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T191850 NZ_CP091964:50748-51089 [Leptospira noguchii]
TTGGTAAAGAATAGAAATTATACTCCTGAAAAAGGAGATATTGTGTGGCTGAACTTTACTCCTCAAGCAGGTCATGAACA
GAAAGGTCGTAGACCTGCACTTGTTTTATCGCCTAAGGAATATAATTCAAAGACAGGGTTAGCAATATTTTGTCCAATTA
CCAGTAAAATTAAAGGTTATCCATTCGAAGTGTTGATTAAATCAAAAAAAATCGATGGTGTAATTCTTTCTGATCAAGTA
AAAAACTTAGATTGGACTATCCGTGAAGCAGAATTTATTGAATCAATAAATAAAGTTTCTTTAAAAGAAGTTTTAGATAA
TATTAAATTATTAATATTCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11815.37 Da
• Isoelectric Point: 6.9457

>AT191850 WP_064793340.1 NZ_CP069815:4673848-4674165 [Raoultella planticola]
MSNTTWGLQRDITPRLGARLVQEGNRLHYLDDRASITGKFSDAQCIKLDVVFPHFISQIESVLTTGELNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT191850 NZ_CP091964:50518-50754 [Leptospira noguchii]
ATGGAATCAATTATTCAAAAATGGGGTAATAGCTTAGGAATTAGAATTCCAAAAGCAATGGCAACGGAATTAGAGTTAAA
TGATGGAAGCCATGTTGAATTACAATATGAAGGAGATAAGATTGTAATATATCCAATGAAAAAAGCTTCATTGGAAGATA
AACTTTCTAAAATTACGAAACAAAATCTTCACTCTGAAATATCTACTGGTAATTCTATAGGCAATGAAGCTTGGTAA

Structures

Toxin

• AlphaFold DB: A0A443VFJ3

Antitoxin

• AlphaFold DB: A0A443VFG7