Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 1743781..1744451 Replicon chromosome
Accession NZ_CP069810
Organism Cupriavidus oxalaticus strain Ox1_mCherry

Toxin (Protein)


Gene name vapC Uniprot ID A0A375G4I0
Locus tag JTE91_RS20445 Protein ID WP_063237843.1
Coordinates 1744032..1744451 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A375FZF0
Locus tag JTE91_RS20440 Protein ID WP_063237844.1
Coordinates 1743781..1744035 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTE91_RS20410 1739531..1740355 + 825 WP_063237847.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein -
JTE91_RS20415 1740339..1741289 + 951 WP_063237846.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
JTE91_RS20420 1741444..1741575 + 132 Protein_1572 DUF2274 domain-containing protein -
JTE91_RS20425 1741716..1742444 + 729 WP_063237845.1 hypothetical protein -
JTE91_RS20430 1742533..1742775 + 243 WP_174544844.1 hypothetical protein -
JTE91_RS20435 1742788..1743345 + 558 WP_174544843.1 hypothetical protein -
JTE91_RS20440 1743781..1744035 + 255 WP_063237844.1 Arc family DNA-binding protein Antitoxin
JTE91_RS20445 1744032..1744451 + 420 WP_063237843.1 type II toxin-antitoxin system VapC family toxin Toxin
JTE91_RS20450 1744557..1745186 + 630 WP_084254486.1 2'-5' RNA ligase family protein -
JTE91_RS20455 1745238..1746428 - 1191 WP_063237842.1 3-oxoadipate enol-lactonase -
JTE91_RS20460 1746466..1747854 - 1389 WP_084254485.1 3-carboxy-cis,cis-muconate cycloisomerase -
JTE91_RS20465 1747913..1748482 - 570 WP_063237840.1 protocatechuate 3,4-dioxygenase subunit alpha -
JTE91_RS20470 1748487..1749188 - 702 WP_063237839.1 protocatechuate 3,4-dioxygenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1741716..1751300 9584


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

Arc-like DNA binding domain

(9-46)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14836.02 Da        Isoelectric Point: 5.6468

>T191827 WP_063237843.1 NZ_CP069810:1744032-1744451 [Cupriavidus oxalaticus]
MIVLDTNVVSEAMKPEPSPAVQAWLNEQVAETLYLSSVTLAELLFGIGALPNGKRKKGLGEALDGLLELFGERVLMFDTE
AARHYAELAAKARTAGKGFPTPHGYIGAIAASKGFIVATRDTSPFEAAGLTVINPWNHQ

Download         Length: 420 bp

>T191827 NZ_CP091925:c4110499-4110212 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAACAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9450.83 Da        Isoelectric Point: 7.2071

>AT191827 WP_063237844.1 NZ_CP069810:1743781-1744035 [Cupriavidus oxalaticus]
MAILTVRNVPDEVHRALRLRAAEHGRSTEAEVRAILESAVKSEKRIRMGDALAELGRRVGLTNDDFAVLDQVRDKVPAEP
MRFE

Download         Length: 255 bp

>AT191827 NZ_CP091925:c4110225-4109923 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A375G4I0

Antitoxin

Source ID Structure
AlphaFold DB A0A375FZF0

References