Detailed information of TA system TA191827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1743781..1744451
• Replicon: chromosome
• Accession: NZ_CP069810
• Organism: Cupriavidus oxalaticus strain Ox1_mCherry

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A375G4I0
• Locus tag: JTE91_RS20445
• Protein ID: WP_063237843.1
• Coordinates: 1744032..1744451 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A375FZF0
• Locus tag: JTE91_RS20440
• Protein ID: WP_063237844.1
• Coordinates: 1743781..1744035 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JTE91_RS20410	1739531..1740355	+	825	WP_063237847.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
JTE91_RS20415	1740339..1741289	+	951	WP_063237846.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JTE91_RS20420	1741444..1741575	+	132	Protein_1572	DUF2274 domain-containing protein	-
JTE91_RS20425	1741716..1742444	+	729	WP_063237845.1	hypothetical protein	-
JTE91_RS20430	1742533..1742775	+	243	WP_174544844.1	hypothetical protein	-
JTE91_RS20435	1742788..1743345	+	558	WP_174544843.1	hypothetical protein	-
JTE91_RS20440	1743781..1744035	+	255	WP_063237844.1	Arc family DNA-binding protein	Antitoxin
JTE91_RS20445	1744032..1744451	+	420	WP_063237843.1	type II toxin-antitoxin system VapC family toxin	Toxin
JTE91_RS20450	1744557..1745186	+	630	WP_084254486.1	2'-5' RNA ligase family protein	-
JTE91_RS20455	1745238..1746428	-	1191	WP_063237842.1	3-oxoadipate enol-lactonase	-
JTE91_RS20460	1746466..1747854	-	1389	WP_084254485.1	3-carboxy-cis,cis-muconate cycloisomerase	-
JTE91_RS20465	1747913..1748482	-	570	WP_063237840.1	protocatechuate 3,4-dioxygenase subunit alpha	-
JTE91_RS20470	1748487..1749188	-	702	WP_063237839.1	protocatechuate 3,4-dioxygenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1741716..1751300	9584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14836.02 Da
• Isoelectric Point: 5.6468

>T191827 WP_063237843.1 NZ_CP069810:1744032-1744451 [Cupriavidus oxalaticus]
MIVLDTNVVSEAMKPEPSPAVQAWLNEQVAETLYLSSVTLAELLFGIGALPNGKRKKGLGEALDGLLELFGERVLMFDTE
AARHYAELAAKARTAGKGFPTPHGYIGAIAASKGFIVATRDTSPFEAAGLTVINPWNHQ

Nucleotide

• Length: 420 bp

>T191827 NZ_CP091925:c4110499-4110212 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAACAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9450.83 Da
• Isoelectric Point: 7.2071

>AT191827 WP_063237844.1 NZ_CP069810:1743781-1744035 [Cupriavidus oxalaticus]
MAILTVRNVPDEVHRALRLRAAEHGRSTEAEVRAILESAVKSEKRIRMGDALAELGRRVGLTNDDFAVLDQVRDKVPAEP
MRFE

Nucleotide

• Length: 255 bp

>AT191827 NZ_CP091925:c4110225-4109923 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A375G4I0

Antitoxin

• AlphaFold DB: A0A375FZF0