191825

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 1009799..1010339 Replicon chromosome
Accession NZ_CP069809
Organism Cupriavidus oxalaticus strain Ox1_mCherry

Toxin (Protein)


Gene name brnT Uniprot ID A0A375GHM6
Locus tag JTE91_RS04430 Protein ID WP_063240620.1
Coordinates 1010070..1010339 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID A0A375GNI1
Locus tag JTE91_RS04425 Protein ID WP_063240621.1
Coordinates 1009799..1010086 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTE91_RS04405 1005749..1006120 - 372 WP_063240625.1 EamA family transporter -
JTE91_RS04410 1006140..1007879 - 1740 WP_063240624.1 glycosyltransferase family 39 protein -
JTE91_RS04415 1008281..1009183 + 903 WP_063240623.1 hypothetical protein -
JTE91_RS04420 1009253..1009561 - 309 WP_063240622.1 hypothetical protein -
JTE91_RS04425 1009799..1010086 - 288 WP_063240621.1 BrnA antitoxin family protein Antitoxin
JTE91_RS04430 1010070..1010339 - 270 WP_063240620.1 BrnT family toxin Toxin
JTE91_RS04435 1010518..1011627 + 1110 WP_063240619.1 acyltransferase -
JTE91_RS04440 1011861..1013099 + 1239 WP_063240618.1 GTP cyclohydrolase II -
JTE91_RS04445 1013096..1014418 + 1323 WP_063240617.1 URC4/urg3 family protein -
JTE91_RS04450 1014518..1015228 + 711 WP_063240616.1 uracil phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1009253..1019135 9882


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-90)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10139.59 Da        Isoelectric Point: 8.0323

>T191825 WP_063240620.1 NZ_CP069809:c1010339-1010070 [Cupriavidus oxalaticus]
MDITFDVAKDAANLSKHGLSLRVAAQLDWPAVMAGVDTRRDYREVREIGFGVIADRLYCVVFTQRGDAMHIISLRKANQR
EVRNYVEQT

Download         Length: 270 bp

>T191825 NZ_CP091925:c3761882-3761484 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10721.20 Da        Isoelectric Point: 8.5541

>AT191825 WP_063240621.1 NZ_CP069809:c1010086-1009799 [Cupriavidus oxalaticus]
MSSKPKIVMPTDEEDAAINRGIATDPDTYEVSADEMMAMKPLGKRGRGRPRLGNTKELVSIRYDAEVLEAFRASGEGWQT
RMNSALKEWLRTHRP

Download         Length: 288 bp

>AT191825 NZ_CP091925:c3762178-3761885 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A375GHM6

Antitoxin

Source ID Structure
AlphaFold DB A0A375GNI1

References