Detailed information of TA system TA191817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2134651..2135180
• Replicon: chromosome
• Accession: NZ_CP069799
• Organism: Staphylococcus aureus strain 014S_SA

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JS513_RS10670
• Protein ID: WP_000621175.1
• Coordinates: 2134651..2135013 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JS513_RS10675
• Protein ID: WP_000948331.1
• Coordinates: 2135010..2135180 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JS513_RS10650	2131629..2132399	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
JS513_RS10655	2132374..2132853	-	480	WP_001190830.1	anti-sigma B factor RsbW	-
JS513_RS10660	2132855..2133181	-	327	WP_001052491.1	anti-sigma factor antagonist	-
JS513_RS10665	2133300..2134301	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JS513_RS10670	2134651..2135013	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JS513_RS10675	2135010..2135180	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JS513_RS10680	2135265..2136413	-	1149	WP_001281145.1	alanine racemase	-
JS513_RS10685	2136479..2136838	-	360	WP_000581200.1	holo-ACP synthase	-
JS513_RS10690	2136842..2137333	-	492	WP_001205910.1	PH domain-containing protein	-
JS513_RS10695	2137320..2138903	-	1584	WP_001294626.1	PH domain-containing protein	-
JS513_RS10700	2138896..2139375	-	480	WP_001287088.1	hypothetical protein	-
JS513_RS10705	2139584..2140144	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T191817 WP_000621175.1 NZ_CP069799:c2135013-2134651 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T191817 NZ_CP091925:1998515-1998618 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT191817 WP_000948331.1 NZ_CP069799:c2135180-2135010 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT191817 NZ_CP091925:c1998656-1998385 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1