Detailed information of TA system TA191801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1121615..1122060
• Replicon: chromosome
• Accession: NZ_CP069798
• Organism: Paralysiella testudinis strain 26B

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JQU52_RS05765
• Protein ID: WP_230340183.1
• Coordinates: 1121842..1122060 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: JQU52_RS05760
• Protein ID: WP_230340173.1
• Coordinates: 1121615..1121845 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQU52_RS05730 (JQU52_05760)	1116849..1117532	+	684	WP_230340178.1	S24 family peptidase	-
JQU52_RS05740 (JQU52_05770)	1118647..1118817	+	171	WP_230340179.1	hypothetical protein	-
JQU52_RS05745 (JQU52_05775)	1119178..1119786	-	609	WP_230340180.1	hypothetical protein	-
JQU52_RS05750 (JQU52_05780)	1119971..1120165	-	195	WP_230340181.1	Cro/CI family transcriptional regulator	-
JQU52_RS05755 (JQU52_05785)	1120270..1120917	+	648	WP_230340182.1	S24 family peptidase	-
JQU52_RS05760 (JQU52_05790)	1121615..1121845	+	231	WP_230340173.1	antitoxin MazE family protein	Antitoxin
JQU52_RS05765 (JQU52_05795)	1121842..1122060	+	219	WP_230340183.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JQU52_RS05775 (JQU52_05805)	1122373..1123263	+	891	WP_230340184.1	hypothetical protein	-
JQU52_RS05780 (JQU52_05810)	1123282..1123977	+	696	WP_230340185.1	hypothetical protein	-
JQU52_RS05785 (JQU52_05815)	1124125..1124670	+	546	WP_230340186.1	TrbM/KikA/MpfK family conjugal transfer protein	-
JQU52_RS05790 (JQU52_05820)	1124618..1125604	+	987	WP_230340187.1	conjugal transfer protein TraL	-
JQU52_RS05795 (JQU52_05825)	1125582..1126016	+	435	WP_230340188.1	hypothetical protein	-
JQU52_RS05800 (JQU52_05830)	1126029..1126283	+	255	WP_230340189.1	toxin PIN	-
JQU52_RS05805 (JQU52_05835)	1126329..1126553	+	225	WP_230340190.1	hypothetical protein	-
JQU52_RS05810 (JQU52_05840)	1126566..1126895	+	330	WP_230340191.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1062617..1127893	65276

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-65)

Antitoxin

• Antitoxin MazE-like (7-68)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8039.40 Da
• Isoelectric Point: 10.1054

>T191801 WP_230340183.1 NZ_CP069798:1121842-1122060 [Paralysiella testudinis]
MKRGDLITVAIQGDYGKLRPALVVQSDVFAGHTSICILPLTSDLHEAPLFRILLTPSSSNGLTKNHRLWWIR

Nucleotide

• Length: 219 bp

>T191801 NZ_CP091913:3239825-3240109 [Nostoc sp. UHCC 0870]
GTGATTGTAAGTTTCAAATCTGAAGAGACAAAGCTCATTTTTGATGGCTTTATATCTGCTGAATACCCGCCAGATATTCA
AAAAGTTGCTTTACGAAAGCTGCTGATGCTGAATGCAGCAACATCAATTCAAGACTTGCGCGTTCCACCAGGTAATCGAT
TGGAAAAGCTAGTTGGGGACAGACAAGGACAATATAGCATTCGTATTAATGACCAGTGGCGAATCTGCTTTGTCTGGAGT
AATGAAAATAATGCGGATGAAGTTGAAATTGTAGACTACCACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8492.66 Da
• Isoelectric Point: 7.3207

>AT191801 WP_230340173.1 NZ_CP069798:1121615-1121845 [Paralysiella testudinis]
MMSNSASRVQQHRVRLRHAGLRPIQIWVPDTRRPGFAAEAHRQIALVAQGDRGDAQLAVMMDEALMNLADSGEWEA

Nucleotide

• Length: 231 bp

>AT191801 NZ_CP091913:3240123-3240431 [Nostoc sp. UHCC 0870]
ATGAATAGTAATTGTTTACCAAATATCCATCCAGGCGAAATTCTGCAACTAGAATTTTTAGAGCCATTGAATATTACCCC
TTATCGGTTGAGTAAAGATATAGGTGTCACTCAGACAAGAATTGGCGAAATTTTATCAGGTAAACGCAGTATTACAGCAG
ATACAGCTTTGCGTTTATCGCGTTATTTTGGTAACAGTCCCCAGTTTTGGCTGAATTTGCAAATGCAATATGATTTACGT
CAAGCTTGGGTAGAAAAGGCAGATGTTTACAACCAGATTCCTGTGAATCCGTTTAATAGTGTAGCTTAA