Detailed information of TA system TA191797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1091055..1091689
• Replicon: chromosome
• Accession: NZ_CP069798
• Organism: Paralysiella testudinis strain 26B

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JQU52_RS05565
• Protein ID: WP_230340146.1
• Coordinates: 1091282..1091689 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JQU52_RS05560
• Protein ID: WP_230340145.1
• Coordinates: 1091055..1091282 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQU52_RS05525 (JQU52_05550)	1088076..1088222	+	147	WP_230340138.1	hypothetical protein	-
JQU52_RS05530 (JQU52_05555)	1088176..1088568	+	393	WP_230340139.1	hypothetical protein	-
JQU52_RS05535 (JQU52_05560)	1088645..1089139	+	495	WP_230340140.1	thermonuclease family protein	-
JQU52_RS05540 (JQU52_05565)	1089174..1089371	+	198	WP_230340141.1	hypothetical protein	-
JQU52_RS05545 (JQU52_05575)	1089739..1090074	+	336	WP_230340142.1	hypothetical protein	-
JQU52_RS05550 (JQU52_05580)	1090104..1090457	-	354	WP_230340143.1	hypothetical protein	-
JQU52_RS05555 (JQU52_05585)	1090512..1090940	+	429	WP_230340144.1	single-stranded DNA-binding protein	-
JQU52_RS05560 (JQU52_05590)	1091055..1091282	+	228	WP_230340145.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JQU52_RS05565 (JQU52_05595)	1091282..1091689	+	408	WP_230340146.1	tRNA(fMet)-specific endonuclease VapC	Toxin
JQU52_RS05570 (JQU52_05600)	1091916..1092191	+	276	WP_230340147.1	DUF1778 domain-containing protein	-
JQU52_RS05575 (JQU52_05605)	1092194..1092700	+	507	WP_230340148.1	GNAT family N-acetyltransferase	-
JQU52_RS05580 (JQU52_05610)	1092761..1094761	+	2001	WP_230340149.1	type IV secretory system conjugative DNA transfer family protein	-
JQU52_RS05585 (JQU52_05615)	1094856..1095377	+	522	WP_230340150.1	hypothetical protein	-
JQU52_RS05590 (JQU52_05620)	1095407..1095670	-	264	WP_230340151.1	Txe/YoeB family addiction module toxin	-
JQU52_RS05595 (JQU52_05625)	1095667..1095921	-	255	WP_230340152.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1062617..1127893	65276

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-116)

Antitoxin

• SpoVT-AbrB domain (6-34)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15063.45 Da
• Isoelectric Point: 7.2648

>T191797 WP_230340146.1 NZ_CP069798:1091282-1091689 [Paralysiella testudinis]
MLKYMLDTNICIYTMKNKPEEVRQAFNLHHTQMCISSVVLMELVFGAEKSARAQEAFAAVEGFAARMMVLDFDEAAAYHT
GQIRAELKKAGKPIGSYDCMIAGHARSRGLIVVSNNLREFDCISGLRTENWVAAQ

Nucleotide

• Length: 408 bp

>T191797 NZ_CP091907:75858-76136 [Enterococcus faecalis]
ATGCTGACGATTGAGTACACTTCTATATTCAAAAGAGATTATAAAAGAATGGTGAAAAAACACTACAATATGAAAAAGTT
AGAAAAAGTAGTTAAGTTGCTTGTCACAAACAACCAAGATGAATTGATAAGAAAATATAAAGACCATGCTTTAAAAGGGA
ACTGGAAAGGTTACAGAGAACTACATTTAGATAAAGACTGGTTACTAATTTACAAGATTGACGACAAAAATTTAATCTTA
ACAATGACACGTACAGGAAGTCATGATGAACTTTTGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8472.70 Da
• Isoelectric Point: 5.0338

>AT191797 WP_230340145.1 NZ_CP069798:1091055-1091282 [Paralysiella testudinis]
MQASIFKSNKSQAVRLPKQVAFPEEVKRVEVVVIGRTRVLMPADEVWDTWFAASPVPDDFMSERAQPAAQERDAL

Nucleotide

• Length: 228 bp

>AT191797 NZ_CP091907:75611-75871 [Enterococcus faecalis]
ATGACTGTATCACGACTTAATATTCGGATTGATGGAGATTTAAAAGAGCAAGCAAAAGAAGTTTACAAAGATATGGGAAT
GGATTTAACCACAGCTGTTACAATATTTCTAAAGCAAAGTGTGCGAGAACAACGTTTACCTTTCCAACCAAGTAGAGAAC
CCATTGAAAATGTTATTGCTCGCTATGAAGTAGAAAATGGCTTGACAACAAAGGTTGATTCAGTAGCAGACCTGATGGAG
AATTTAAATGCTGACGATTGA