Detailed information of TA system TA191783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 2136427..2137142
• Replicon: chromosome
• Accession: NZ_CP069795
• Organism: Actinobacillus pleuropneumoniae strain A-85/14

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JS559_RS10250
• Protein ID: WP_005599445.1
• Coordinates: 2136427..2136792 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: JS559_RS10255
• Protein ID: WP_005599447.1
• Coordinates: 2136855..2137142 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JS559_RS10235 (JS559_10240)	2132012..2133718	-	1707	WP_237610950.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
JS559_RS10240 (JS559_10245)	2133814..2134095	-	282	WP_012263402.1	DNA-directed RNA polymerase subunit omega	-
JS559_RS10245 (JS559_10250)	2134222..2136303	-	2082	WP_005613379.1	ATP-dependent DNA helicase RecG	-
JS559_RS10250 (JS559_10255)	2136427..2136792	-	366	WP_005599445.1	type II toxin-antitoxin system VapC family toxin	Toxin
JS559_RS10255 (JS559_10260)	2136855..2137142	-	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JS559_RS10260 (JS559_10265)	2137348..2139063	+	1716	WP_237610951.1	proline--tRNA ligase	-
JS559_RS10265	2139129..2139317	-	189	WP_005602684.1	hypothetical protein	-
JS559_RS10270 (JS559_10270)	2139320..2139901	-	582	WP_005606496.1	sigma-70 family RNA polymerase sigma factor	-
JS559_RS10275 (JS559_10275)	2139989..2140945	+	957	WP_237610952.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
JS559_RS10280 (JS559_10280)	2140945..2141538	+	594	WP_005599453.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13974.06 Da
• Isoelectric Point: 9.0549

>T191783 WP_005599445.1 NZ_CP069795:c2136792-2136427 [Actinobacillus pleuropneumoniae]
MDTNVRQWLATIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQAKSFSINNEIALLASEYHIPNK
MDLNDAYIAATAKYHNLVLVTRNLKDFNRCDIRLFNPFEPN

Nucleotide

• Length: 366 bp

>T191783 NZ_CP091904:c2893174-2892833 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGCGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT191783 WP_005599447.1 NZ_CP069795:c2137142-2136855 [Actinobacillus pleuropneumoniae]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT191783 NZ_CP091904:c2893404-2893174 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5