Detailed information of TA system TA191782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2051417..2052045
• Replicon: chromosome
• Accession: NZ_CP069795
• Organism: Actinobacillus pleuropneumoniae strain A-85/14

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JS559_RS09730
• Protein ID: WP_237610933.1
• Coordinates: 2051647..2052045 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B3GYV7
• Locus tag: JS559_RS09725
• Protein ID: WP_005613300.1
• Coordinates: 2051417..2051647 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JS559_RS09715 (JS559_09720)	2048725..2050275	+	1551	WP_005616128.1	class I SAM-dependent methyltransferase	-
JS559_RS09720 (JS559_09725)	2050272..2051054	+	783	WP_237610932.1	HincII family type II restriction endonuclease	-
JS559_RS09725 (JS559_09730)	2051417..2051647	+	231	WP_005613300.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JS559_RS09730 (JS559_09735)	2051647..2052045	+	399	WP_237610933.1	tRNA(fMet)-specific endonuclease VapC	Toxin
JS559_RS09735 (JS559_09740)	2052134..2053467	+	1334	WP_237610934.1	IS3 family transposase	-
JS559_RS09740 (JS559_09745)	2053602..2054018	-	417	WP_005599244.1	DUF417 family protein	-
JS559_RS09745 (JS559_09750)	2054255..2055445	-	1191	WP_005599245.1	pentapeptide repeat-containing protein	-
JS559_RS09750 (JS559_09755)	2055498..2056820	-	1323	WP_237610935.1	HslU--HslV peptidase ATPase subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2035019..2055445	20426

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-53)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15119.42 Da
• Isoelectric Point: 7.4195

>T191782 WP_237610933.1 NZ_CP069795:2051647-2052045 [Actinobacillus pleuropneumoniae]
MLTYMLDTNIAIYVIKRRPIEVLDKFNLNSTRLCVSSITAAELYYGAEKSQFPERNMVVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKQGKIIGENDIHIAAHARSEGLVLVTNNLREFERVEGLRLDNWV

Nucleotide

• Length: 399 bp

>T191782 NZ_CP091904:c2395838-2395722 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8625.71 Da
• Isoelectric Point: 4.5358

>AT191782 WP_005613300.1 NZ_CP069795:2051417-2051647 [Actinobacillus pleuropneumoniae]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLSSATVSDDFMTEREISTQAEREEL

Nucleotide

• Length: 231 bp

>AT191782 NZ_CP091904:2395606-2395811 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTTTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Structures

Antitoxin

• AlphaFold DB: A3N328