Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1831480..1832211 Replicon chromosome
Accession NZ_CP069795
Organism Actinobacillus pleuropneumoniae strain A-85/14

Toxin (Protein)


Gene name tacT Uniprot ID A0A448U107
Locus tag JS559_RS08645 Protein ID WP_005602211.1
Coordinates 1831480..1831947 (-) Length 156 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID B0BRD3
Locus tag JS559_RS08650 Protein ID WP_005619269.1
Coordinates 1831951..1832211 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JS559_RS08620 (JS559_08620) 1826839..1827210 - 372 WP_005598830.1 CrcB family protein -
JS559_RS08625 (JS559_08625) 1827213..1828277 - 1065 WP_011848610.1 MFS transporter -
JS559_RS08630 (JS559_08630) 1828372..1829481 + 1110 WP_012263272.1 anhydro-N-acetylmuramic acid kinase -
JS559_RS08635 (JS559_08635) 1829535..1830449 + 915 WP_237610872.1 N-acetylmuramic acid 6-phosphate etherase -
JS559_RS08640 (JS559_08640) 1830516..1831397 - 882 WP_237610873.1 50S ribosomal protein L11 methyltransferase -
JS559_RS08645 (JS559_08645) 1831480..1831947 - 468 WP_005602211.1 GNAT family N-acetyltransferase Toxin
JS559_RS08650 (JS559_08650) 1831951..1832211 - 261 WP_005619269.1 DUF1778 domain-containing protein Antitoxin
JS559_RS08655 (JS559_08655) 1832332..1833792 - 1461 WP_237593341.1 metalloprotease TldD -
JS559_RS08660 (JS559_08660) 1833922..1835181 + 1260 WP_237610874.1 tRNA lysidine(34) synthetase TilS -
JS559_RS08665 (JS559_08665) 1835208..1835420 - 213 WP_237593654.1 DUF5389 family protein -
JS559_RS08670 (JS559_08670) 1835500..1836393 - 894 WP_005598850.1 site-specific tyrosine recombinase XerD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(22-142)

Antitoxin

Vibrio phage ICP1, Orf50

(6-80)


Sequences

Toxin        


Download         Length: 156 a.a.        Molecular weight: 17192.19 Da        Isoelectric Point: 8.9312

>T191781 WP_005602211.1 NZ_CP069795:c1831947-1831480 [Actinobacillus pleuropneumoniae]
MHAPELLSEQHIVRYFQCGEVVLDQWLQRTALKNQQNNASKTFVVCDENKQVMGFYCLSAGSVSHQFVAGALRRNMPDPI
PVIILGRLAVDCSAQGKQLGVSMLKDAVLKSKTVANQIGVKALLVHALNPQAKQFYLKYGFSCSPIDEMVLMLKL

Download         Length: 468 bp

>T191781 NZ_CP091904:c469141-468797 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTGGAAGAATATGAAGTAGAGTTAGTTTTTGCTCCAATAAATAAGCGCGCGTGTTATGA
GTCGGTCAAAAGGATAATTTTCGTAAATCAAAATTTATCTATCGAAGAACAAGAAGAAGCTATATTCCATGAGTTTAAAC
ATGTGGTTTCCCATTCGGATTACATGGAGTTATATAAAATCCCTTCTTTTAGAAATAAAATGGAAGCTGAGGCAGATCAT
CATATGTTTAAATGCCTTATTGAAAAACATGACGGGCAATTTAATTATTCTAACGTGATTACTCATTACAATTTAAAGAT
GGGACAGGAAAATTATTTAAAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9796.14 Da        Isoelectric Point: 4.4883

>AT191781 WP_005619269.1 NZ_CP069795:c1832211-1831951 [Actinobacillus pleuropneumoniae]
MRTAPINLRAMPDQRDLIDYAASILGKTRSDFMLETACQAAQNVILDRTVFQLNEQAFEQFNRLLEQPTADNPGLDKLLN
TKAVWE

Download         Length: 261 bp

>AT191781 NZ_CP091904:c469482-469159 [Enterococcus faecalis]
ATGACTGTATTTGATAGAGTTAAAAAATTAGCAGATAGTCAAAAAATATCTATTGTGGAACTTGAAGAAAAGTTAAATTT
TAGTCGAAATTCATTGTATGCTTGGAAAAAAAGCAAGCCTTCTATCGATAAATTAGAAGCTGTCGCAAATTATTTTGGAG
TTTCAACGGATTATTTATTGGGTCGTGAAATCTCTAATAAACCAAAGCAATCTGATGACTTGGATGAAATTTTAGACAAT
GTGATGAGTTTTGATGGTGAGCCTTTGGATGATCATGATCGGGAAGTTATTCGTGCTTACTTAAAAGGAAGATTCGGAAA
ATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A448U107

Antitoxin

Source ID Structure
AlphaFold DB A3N2I7

References