191777

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1340924..1341840 Replicon chromosome
Accession NZ_CP069789
Organism Bacillus subtilis strain BIM B-569G

Toxin (Protein)


Gene name spoIISA Uniprot ID -
Locus tag JR441_RS07050 Protein ID WP_089172519.1
Coordinates 1341094..1341840 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag JR441_RS07045 Protein ID WP_003232646.1
Coordinates 1340924..1341094 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR441_RS07005 1336638..1336967 + 330 WP_198878712.1 XkdW family protein -
JR441_RS07010 1336964..1337128 + 165 WP_047182493.1 XkdX family protein -
JR441_RS07015 1337175..1338014 + 840 WP_198878713.1 phage-like element PBSX protein XepA -
JR441_RS07020 1338067..1338336 + 270 WP_015252265.1 hemolysin XhlA family protein -
JR441_RS07025 1338349..1338612 + 264 WP_014479566.1 phage holin -
JR441_RS07030 1338625..1339521 + 897 WP_198878714.1 N-acetylmuramoyl-L-alanine amidase -
JR441_RS07035 1339598..1340689 + 1092 WP_198878715.1 acyltransferase family protein -
JR441_RS07040 1340686..1340838 - 153 WP_131228329.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
JR441_RS07045 1340924..1341094 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
JR441_RS07050 1341094..1341840 - 747 WP_089172519.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
JR441_RS07055 1341950..1342951 - 1002 WP_014479569.1 inorganic phosphate transporter -
JR441_RS07060 1342964..1343581 - 618 WP_003218470.1 DUF47 domain-containing protein -
JR441_RS07065 1343857..1345173 - 1317 WP_015252262.1 serine/threonine exchanger -
JR441_RS07070 1345562..1346512 + 951 WP_003245086.1 ring-cleaving dioxygenase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-238)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29121.60 Da        Isoelectric Point: 4.6191

>T191777 WP_089172519.1 NZ_CP069789:c1341840-1341094 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMFAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T191777 NZ_CP091902:79598-80461 [Enterococcus faecalis]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT191777 WP_003232646.1 NZ_CP069789:c1341094-1340924 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT191777 NZ_CP091902:79324-79596 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References