Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 513401..514037 Replicon chromosome
Accession NZ_CP069789
Organism Bacillus subtilis strain BIM B-569G

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag JR441_RS02610 Protein ID WP_003156187.1
Coordinates 513687..514037 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag JR441_RS02605 Protein ID WP_003225183.1
Coordinates 513401..513682 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR441_RS02585 509761..510360 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
JR441_RS02590 510455..510820 + 366 WP_015252768.1 holo-ACP synthase -
JR441_RS02595 510986..512002 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
JR441_RS02600 512116..513285 + 1170 WP_015252766.1 alanine racemase -
JR441_RS02605 513401..513682 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
JR441_RS02610 513687..514037 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JR441_RS02615 514153..514977 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
JR441_RS02620 514982..515347 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
JR441_RS02625 515351..515752 + 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
JR441_RS02630 515764..516771 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
JR441_RS02635 516833..517162 + 330 WP_080528943.1 anti-sigma factor antagonist RsbV -
JR441_RS02640 517159..517641 + 483 WP_003234299.1 anti-sigma B factor RsbW -
JR441_RS02645 517607..518395 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
JR441_RS02650 518395..518994 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T191776 WP_003156187.1 NZ_CP069789:513687-514037 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T191776 NZ_CP091902:c70879-70538 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGGAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACAATGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCCACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGATTTTACAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT191776 WP_003225183.1 NZ_CP069789:513401-513682 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT191776 NZ_CP091902:c71109-70879 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAACAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGATCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGTATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References