Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 150882..151407 Replicon plasmid pTA13-1
Accession NZ_CP069713
Organism Escherichia coli strain EA13

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag JSQ77_RS23595 Protein ID WP_001159868.1
Coordinates 150882..151187 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JSQ77_RS23600 Protein ID WP_000813634.1
Coordinates 151189..151407 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ77_RS23570 146006..146761 - 756 WP_000852146.1 replication initiation protein RepE -
JSQ77_RS23575 146935..147360 + 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
JSQ77_RS23580 147357..147707 + 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
JSQ77_RS23585 147738..149351 + 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
JSQ77_RS23590 150075..150881 - 807 WP_000016982.1 site-specific integrase -
JSQ77_RS23595 150882..151187 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
JSQ77_RS23600 151189..151407 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JSQ77_RS23605 151975..152487 + 513 WP_000151784.1 hypothetical protein -
JSQ77_RS23610 152521..153654 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
JSQ77_RS23615 153821..154594 - 774 WP_000905949.1 hypothetical protein -
JSQ77_RS23620 154607..155107 - 501 WP_000528931.1 hypothetical protein -
JSQ77_RS23625 155372..155602 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JSQ77_RS23630 155599..156015 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / erm(42) / tet(A) / dfrA14 / mph(A) / aac(3)-IId / blaTEM-1B / sul3 / aph(3')-Ia / sitABCD iutA / iucD / iucC / iucB / iucA 1..182585 182585


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T191771 WP_001159868.1 NZ_CP069713:c151187-150882 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T191771 NZ_CP091901:c2843954-2843853 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT191771 WP_000813634.1 NZ_CP069713:c151407-151189 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT191771 NZ_CP091901:2843729-2843908 [Enterococcus faecalis]
TGAAAAGAGAGATATGCTACTACATACCTCTCCTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGT
TTTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACC
GAAAATCAGTAGTGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References