Detailed information of TA system TA191765

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4030860..4031695
• Replicon: chromosome
• Accession: NZ_CP069712
• Organism: Escherichia coli strain EA13

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LDL9
• Locus tag: JSQ77_RS19400
• Protein ID: WP_001094444.1
• Coordinates: 4030860..4031237 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7LDN9
• Locus tag: JSQ77_RS19405
• Protein ID: WP_001285607.1
• Coordinates: 4031327..4031695 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSQ77_RS19370	4026073..4027179	+	1107	WP_001353974.1	N-acetylneuraminate epimerase	-
JSQ77_RS19375	4027244..4028224	+	981	WP_001295601.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
JSQ77_RS19380	4028232..4028882	-	651	WP_001037966.1	HNH endonuclease	-
JSQ77_RS19385	4029927..4030073	-	147	WP_001290178.1	hypothetical protein	-
JSQ77_RS19390	4030158..4030355	-	198	WP_001327226.1	DUF957 domain-containing protein	-
JSQ77_RS19395	4030375..4030863	-	489	WP_000761685.1	hypothetical protein	-
JSQ77_RS19400	4030860..4031237	-	378	WP_001094444.1	TA system toxin CbtA family protein	Toxin
JSQ77_RS19405	4031327..4031695	-	369	WP_001285607.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JSQ77_RS19410	4031775..4031996	-	222	WP_000692350.1	DUF987 domain-containing protein	-
JSQ77_RS19415	4032083..4032559	-	477	WP_001384029.1	RadC family protein	-
JSQ77_RS19420	4032574..4033059	-	486	WP_000213722.1	antirestriction protein	-
JSQ77_RS19425	4033151..4033969	-	819	WP_085668755.1	DUF945 domain-containing protein	-
JSQ77_RS19430	4034059..4034268	-	210	WP_032150870.1	DUF905 family protein	-
JSQ77_RS19435	4034298..4034975	-	678	WP_001097301.1	hypothetical protein	-
JSQ77_RS19440	4035123..4035803	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	4020568..4097882	77314

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14087.02 Da
• Isoelectric Point: 7.3523

>T191765 WP_001094444.1 NZ_CP069712:c4031237-4030860 [Escherichia coli]
MNTLPDTHVREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SVCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T191765 NZ_CP091900:c3244-2849 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCGAATCGCATGACAGAAGCACTTATTAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGGTTCTGTTGCAAAGTTTTAAATCTACTATCAAATAAGGTAGAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13689.53 Da
• Isoelectric Point: 6.4669

>AT191765 WP_001285607.1 NZ_CP069712:c4031695-4031327 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT191765 NZ_CP091900:c3518-3246 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Toxin

• AlphaFold DB: B1LDL9

Antitoxin

• AlphaFold DB: A0A1M0JLU8