Detailed information of TA system TA191764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3994616..3995192
• Replicon: chromosome
• Accession: NZ_CP069712
• Organism: Escherichia coli strain EA13

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: JSQ77_RS19205
• Protein ID: WP_001297643.1
• Coordinates: 3994905..3995192 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: JSQ77_RS19200
• Protein ID: WP_112901517.1
• Coordinates: 3994616..3994918 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSQ77_RS19185	3991212..3993362	+	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-
JSQ77_RS19190	3993412..3993615	+	204	WP_000467859.1	YbdD/YjiX family protein	-
JSQ77_RS19195	3993626..3994582	+	957	WP_001297640.1	GTPase	-
JSQ77_RS19200	3994616..3994918	-	303	WP_112901517.1	BrnA antitoxin family protein	Antitoxin
JSQ77_RS19205	3994905..3995192	-	288	WP_001297643.1	BrnT family toxin	Toxin
JSQ77_RS19210	3995391..3996088	-	698	Protein_3751	IS1 family transposase	-
JSQ77_RS19215	3996145..3996240	+	96	Protein_3752	dienelactone hydrolase family protein	-
JSQ77_RS19220	3996386..3997618	+	1233	WP_063856283.1	multidrug efflux MFS transporter MdtM	-
JSQ77_RS19225	3997659..3998939	+	1281	WP_001037400.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3995391..3995600	209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T191764 WP_001297643.1 NZ_CP069712:c3995192-3994905 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T191764 NZ_CP091899:2947671-2947814 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCGACTTTAATCTTCGGAATCCTAAAGGTTGTTAAAGAAGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11478.96 Da
• Isoelectric Point: 8.4751

>AT191764 WP_112901517.1 NZ_CP069712:c3994918-3994616 [Escherichia coli]
MSMVKHKLGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT191764 NZ_CP091899:c2948004-2947746 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTTAGAAAAATAAGAGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTATA
ATAGCATTGAAAAGAGAGATATGCTGTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCGATTTTGTTACAA
AAAATAACCGTACTCAGTCAAAGTAAACGGTTATTTTTTCTTGTCTTCTTTAACAACCTTTAGGATTCCGAAGATTAAAG
TCGCGATAAAAGAACCGAA

Structures

Toxin

• AlphaFold DB: A0A829GEV9