Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1013039..1013622 Replicon chromosome
Accession NZ_CP069712
Organism Escherichia coli strain EA13

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag JSQ77_RS04835 Protein ID WP_000254738.1
Coordinates 1013287..1013622 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1V2T5M9
Locus tag JSQ77_RS04830 Protein ID WP_000581941.1
Coordinates 1013039..1013287 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ77_RS04820 1009378..1010679 + 1302 WP_088213432.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
JSQ77_RS04825 1010727..1012961 + 2235 WP_000226815.1 GTP pyrophosphokinase -
JSQ77_RS04830 1013039..1013287 + 249 WP_000581941.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JSQ77_RS04835 1013287..1013622 + 336 WP_000254738.1 endoribonuclease MazF Toxin
JSQ77_RS04840 1013693..1014484 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
JSQ77_RS04845 1014712..1016349 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JSQ77_RS04850 1016437..1017735 + 1299 WP_000036723.1 phosphopyruvate hydratase -
JSQ77_RS04855 1017795..1018475 - 681 Protein_950 TPM domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T191747 WP_000254738.1 NZ_CP069712:1013287-1013622 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T191747 NZ_CP091897:c300306-300211 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9385.76 Da        Isoelectric Point: 4.4665

>AT191747 WP_000581941.1 NZ_CP069712:1013039-1013287 [Escherichia coli]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT191747 NZ_CP091897:300111-300176 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATCAGTAGTAGGGTGTCTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2T5M9

References