Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4273675..4274293 Replicon chromosome
Accession NZ_CP069710
Organism Escherichia coli strain EA2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JSQ74_RS20430 Protein ID WP_001291435.1
Coordinates 4273675..4273893 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JSQ74_RS20435 Protein ID WP_000344800.1
Coordinates 4273919..4274293 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ74_RS20395 4268964..4269536 + 573 WP_000779831.1 YbaY family lipoprotein -
JSQ74_RS20400 4269567..4269878 - 312 WP_000409911.1 MGMT family protein -
JSQ74_RS20410 4270257..4270610 + 354 WP_000878140.1 DUF1428 domain-containing protein -
JSQ74_RS20415 4270652..4272202 - 1551 WP_021553404.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JSQ74_RS20420 4272366..4272836 - 471 WP_000136192.1 YlaC family protein -
JSQ74_RS20425 4272952..4273503 - 552 WP_000102568.1 maltose O-acetyltransferase -
JSQ74_RS20430 4273675..4273893 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JSQ74_RS20435 4273919..4274293 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JSQ74_RS20440 4274839..4277988 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
JSQ74_RS20445 4278011..4279204 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T191741 WP_001291435.1 NZ_CP069710:c4273893-4273675 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T191741 NZ_CP091895:614682-614798 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT191741 WP_000344800.1 NZ_CP069710:c4274293-4273919 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT191741 NZ_CP091895:c614914-614709 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References