Detailed information of TA system TA191740

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4085500..4086334
• Replicon: chromosome
• Accession: NZ_CP069710
• Organism: Escherichia coli strain EA2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2X7CAN1
• Locus tag: JSQ74_RS19545
• Protein ID: WP_039025883.1
• Coordinates: 4085957..4086334 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JSQ74_RS19540
• Protein ID: WP_001285578.1
• Coordinates: 4085500..4085868 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSQ74_RS19510	4082419..4082874	+	456	WP_000581504.1	hypothetical protein	-
JSQ74_RS19515	4082953..4083186	+	234	WP_001119729.1	DUF905 family protein	-
JSQ74_RS19520	4083286..4084104	+	819	WP_001234620.1	DUF945 domain-containing protein	-
JSQ74_RS19525	4084159..4084644	+	486	WP_044860884.1	antirestriction protein	-
JSQ74_RS19530	4084660..4085136	+	477	WP_001186725.1	RadC family protein	-
JSQ74_RS19535	4085199..4085420	+	222	WP_000692326.1	DUF987 domain-containing protein	-
JSQ74_RS19540	4085500..4085868	+	369	WP_001285578.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
JSQ74_RS19545	4085957..4086334	+	378	WP_039025883.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JSQ74_RS19550	4086331..4086819	+	489	Protein_3812	hypothetical protein	-
JSQ74_RS19555	4086839..4087036	+	198	WP_000772024.1	DUF957 domain-containing protein	-
JSQ74_RS19560	4087133..4087981	+	849	WP_039025884.1	DUF4942 domain-containing protein	-
JSQ74_RS19565	4088074..4089393	-	1320	WP_000144688.1	site-specific integrase	-
JSQ74_RS19570	4089731..4090069	-	339	Protein_3816	LysR family transcriptional regulator	-
JSQ74_RS19575	4090120..4091076	-	957	WP_040091001.1	molybdenum cofactor insertion chaperone PaoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4047165..4089393	42228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14176.22 Da
• Isoelectric Point: 7.8523

>T191740 WP_039025883.1 NZ_CP069710:4085957-4086334 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQLEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T191740 NZ_CP091895:392303-392578 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13607.39 Da
• Isoelectric Point: 6.3147

>AT191740 WP_001285578.1 NZ_CP069710:4085500-4085868 [Escherichia coli]
VSDTLPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT191740 NZ_CP091895:392044-392310 [Enterococcus faecalis]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Structures

Toxin

• AlphaFold DB: A0A2X7CAN1