Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2728434..2729065 Replicon chromosome
Accession NZ_CP069710
Organism Escherichia coli strain EA2

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag JSQ74_RS13245 Protein ID WP_001259385.1
Coordinates 2728790..2729065 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag JSQ74_RS13240 Protein ID WP_000593554.1
Coordinates 2728434..2728793 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ74_RS13215 2724566..2725510 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
JSQ74_RS13220 2725507..2726340 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
JSQ74_RS13225 2726340..2727104 + 765 WP_096261437.1 nickel import ATP-binding protein NikD -
JSQ74_RS13230 2727101..2727907 + 807 WP_096261436.1 nickel import ATP-binding protein NikE -
JSQ74_RS13235 2727913..2728314 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
JSQ74_RS13240 2728434..2728793 - 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JSQ74_RS13245 2728790..2729065 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
JSQ74_RS13250 2729138..2730262 - 1125 WP_004976779.1 ABC transporter permease -
JSQ74_RS13255 2730262..2732997 - 2736 WP_096261435.1 ribosome-associated ATPase/putative transporter RbbA -
JSQ74_RS13260 2732994..2734061 - 1068 WP_096998094.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T191735 WP_001259385.1 NZ_CP069710:c2729065-2728790 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T191735 NZ_CP091892:2838174-2838317 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT191735 WP_000593554.1 NZ_CP069710:c2728793-2728434 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT191735 NZ_CP091892:c2838511-2838249 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGAGGGTCGTTGTGCTATA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATACCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTCTTCTTTAACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References