Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2117689..2118343 Replicon chromosome
Accession NZ_CP069710
Organism Escherichia coli strain EA2

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag JSQ74_RS10170 Protein ID WP_000244781.1
Coordinates 2117689..2118096 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JSQ74_RS10175 Protein ID WP_000354046.1
Coordinates 2118077..2118343 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ74_RS10150 2113646..2115379 - 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -
JSQ74_RS10155 2115385..2116095 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JSQ74_RS10160 2116120..2117016 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JSQ74_RS10165 2117128..2117649 + 522 WP_001055874.1 flavodoxin FldB -
JSQ74_RS10170 2117689..2118096 - 408 WP_000244781.1 protein YgfX Toxin
JSQ74_RS10175 2118077..2118343 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JSQ74_RS10180 2118586..2119566 + 981 WP_000886083.1 tRNA-modifying protein YgfZ -
JSQ74_RS10185 2119762..2120421 - 660 WP_000250274.1 hemolysin III family protein -
JSQ74_RS10190 2120585..2120896 - 312 WP_204084392.1 N(4)-acetylcytidine aminohydrolase -
JSQ74_RS10195 2120941..2122374 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
JSQ74_RS10200 2122431..2123174 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T191732 WP_000244781.1 NZ_CP069710:c2118096-2117689 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T191732 NZ_CP091892:c2713375-2713271 [Enterococcus faecalis]
TTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGGTATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATT
GCTTAAAAATGACAATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT191732 WP_000354046.1 NZ_CP069710:c2118343-2118077 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT191732 NZ_CP091892:2713515-2713713 [Enterococcus faecalis]
TTTTTAGGGAAATGTGCTATAATGAAAACGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGTAGAGCCGTTTAAGA
CGGTGACCGATTGTATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTATTGTCATTTTTAAGCAATT
TCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References