Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1981210..1981793 Replicon chromosome
Accession NZ_CP069710
Organism Escherichia coli strain EA2

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag JSQ74_RS09545 Protein ID WP_000254738.1
Coordinates 1981210..1981545 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag JSQ74_RS09550 Protein ID WP_000581937.1
Coordinates 1981545..1981793 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ74_RS09530 1977097..1978395 - 1299 WP_000036723.1 phosphopyruvate hydratase -
JSQ74_RS09535 1978483..1980120 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JSQ74_RS09540 1980348..1981139 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
JSQ74_RS09545 1981210..1981545 - 336 WP_000254738.1 endoribonuclease MazF Toxin
JSQ74_RS09550 1981545..1981793 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JSQ74_RS09555 1981871..1984105 - 2235 WP_000226815.1 GTP pyrophosphokinase -
JSQ74_RS09560 1984153..1985454 - 1302 WP_096261334.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T191731 WP_000254738.1 NZ_CP069710:c1981545-1981210 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T191731 NZ_CP091892:c2713375-2713271 [Enterococcus faecalis]
TTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGGTATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATT
GCTTAAAAATGACAATAAAAAATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT191731 WP_000581937.1 NZ_CP069710:c1981793-1981545 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT191731 NZ_CP091892:2713152-2713297 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCGATT
TTGTTACAAAAAATAACCGTACTCGATCAAAGTAAACGGTTATTTTTTATTGTCATTTTTAAGCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References