Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 542418..543056 Replicon chromosome
Accession NZ_CP069710
Organism Escherichia coli strain EA2

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JSQ74_RS02630 Protein ID WP_000813794.1
Coordinates 542418..542594 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JSQ74_RS02635 Protein ID WP_001270286.1
Coordinates 542640..543056 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ74_RS02610 538037..539251 - 1215 WP_001324881.1 BenE family transporter YdcO -
JSQ74_RS02615 539304..539840 + 537 WP_000429169.1 DNA-binding transcriptional regulator SutR -
JSQ74_RS02620 539913..541874 + 1962 WP_204084278.1 U32 family peptidase -
JSQ74_RS02625 541966..542196 - 231 WP_157700780.1 YncJ family protein -
JSQ74_RS02630 542418..542594 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JSQ74_RS02635 542640..543056 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JSQ74_RS02640 543135..544541 + 1407 WP_204084279.1 PLP-dependent aminotransferase family protein -
JSQ74_RS02645 544786..545931 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
JSQ74_RS02650 545949..546962 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
JSQ74_RS02655 546963..547904 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 536849..537997 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T191721 WP_000813794.1 NZ_CP069710:542418-542594 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T191721 NZ_CP091890:74982-75092 [Enterococcus faecalis]
GTGTTATTTGTGAAAGATTTAATGTCGTTGGTTATCGCACCGATCTTTGTAGGATTGGTTCTGGAAATGATTTCTCGTGT
GTTGGACGAGGAAGACGATAACCGAAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT191721 WP_001270286.1 NZ_CP069710:542640-543056 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT191721 NZ_CP091890:c75196-75132 [Enterococcus faecalis]
AACGACATTAAATCGTACAAATAACACAAAAAGCAATCCTACGGCGAATAGGATTGCTTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References