Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4148461..4148958 Replicon chromosome
Accession NZ_CP069709
Organism Escherichia coli strain EA7

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JSQ43_RS20610 Protein ID WP_001173343.1
Coordinates 4148461..4148634 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JSQ43_RS20615 Protein ID WP_000398619.1
Coordinates 4148662..4148958 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ43_RS20600 4145131..4146588 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
JSQ43_RS20605 4146825..4148342 + 1518 WP_001295074.1 lysine--tRNA ligase -
JSQ43_RS20610 4148461..4148634 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JSQ43_RS20615 4148662..4148958 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JSQ43_RS20620 4149185..4149457 - 273 WP_000405651.1 N-acetyltransferase -
JSQ43_RS20625 4149469..4149699 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
JSQ43_RS20630 4149880..4151511 + 1632 WP_001216477.1 sensor histidine kinase -
JSQ43_RS20635 4151508..4152227 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4104442..4148958 44516


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T191720 WP_001173343.1 NZ_CP069709:c4148634-4148461 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T191720 NZ_CP091890:c70469-70107 [Enterococcus faecalis]
ATGGTAAAAGTTCCTCATCAAGGTGATATTTTATTGTTGAATACGGCTCCTAGATCAGGACATGAACAAACAGGTAAGAG
ACCGTATATTGTTTTAAGTCATGATATTATTGCAGATTACAGCAATGTTGTTATTGTAGCTCCTATATCAAGTACAAAAA
GAAACTATCCTTTATATGTTTCTATTAATCCTTCTTACGGAATGAAAACATCAGGTAAAGTATTATTAGATCAATTAACC
ACCATCGATTATGAGGCTAGACAATGCGTGTTTTTAGAAACAGCACACGAAAAATTAATCGATGAATTATTGCTAAAAGT
TAGAACCGTATTTCAAAAAGTAAATAAAACAAATAAATTTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT191720 WP_000398619.1 NZ_CP069709:c4148958-4148662 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT191720 NZ_CP091890:c70726-70463 [Enterococcus faecalis]
ATGGTAATGTTATCAATAAAAAAATGGGGCAATAGTAACGGTCTTAGACTTCCTAAATCTGTGATGGAATATCTCCAAAT
TCATACAGAAGATAAAGTGAAAATAACACAGGAGGAATCGAATGGTAAAAAACGATTAATTATTGAAGCTGTGGATTCTG
ATAATGATTTAACAATTGAACAATTGTTTGAAAATTATAAAGAAGAGAAGGTGCATGTAACTATTCAAGATTTAGGAAAT
GCGGTAGGAAATGAAAAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References