Detailed information of TA system TA191719

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4135623..4136421
• Replicon: chromosome
• Accession: NZ_CP069709
• Organism: Escherichia coli strain EA7

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: U9XMP3
• Locus tag: JSQ43_RS20555
• Protein ID: WP_000854735.1
• Coordinates: 4136044..4136421 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A067H947
• Locus tag: JSQ43_RS20550
• Protein ID: WP_001285415.1
• Coordinates: 4135623..4135997 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSQ43_RS20515	4131554..4132234	+	681	WP_001282919.1	WYL domain-containing protein	-
JSQ43_RS20520	4132382..4133059	+	678	WP_063100886.1	hypothetical protein	-
JSQ43_RS20525	4133065..4133298	+	234	WP_001278287.1	DUF905 family protein	-
JSQ43_RS20530	4133388..4134206	+	819	WP_001175148.1	DUF945 domain-containing protein	-
JSQ43_RS20535	4134288..4134767	+	480	WP_000844100.1	antirestriction protein	-
JSQ43_RS20540	4134783..4135259	+	477	WP_001366855.1	RadC family protein	-
JSQ43_RS20545	4135322..4135543	+	222	WP_000692309.1	DUF987 domain-containing protein	-
JSQ43_RS20550	4135623..4135997	+	375	WP_001285415.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JSQ43_RS20555	4136044..4136421	+	378	WP_000854735.1	TA system toxin CbtA family protein	Toxin
JSQ43_RS20560	4136418..4136906	+	489	WP_000761676.1	hypothetical protein	-
JSQ43_RS20565	4136918..4137115	+	198	WP_000839291.1	DUF957 domain-containing protein	-
JSQ43_RS20570	4137200..4138066	+	867	WP_001280433.1	DUF4942 domain-containing protein	-
JSQ43_RS20575	4138138..4138401	+	264	WP_001143297.1	type II toxin-antitoxin system ParD family antitoxin	-
JSQ43_RS20580	4138398..4138724	+	327	WP_000779483.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JSQ43_RS20585	4139607..4141145	+	1539	WP_024218489.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4104442..4148958	44516

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14061.07 Da
• Isoelectric Point: 8.2904

>T191719 WP_000854735.1 NZ_CP069709:4136044-4136421 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T191719 NZ_CP091889:3064880-3065023 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13663.32 Da
• Isoelectric Point: 5.8640

>AT191719 WP_001285415.1 NZ_CP069709:4135623-4135997 [Escherichia coli]
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT191719 NZ_CP091889:c3065217-3064955 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGAGGGTCGTTGTGCTATA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATACCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTCTTCTTTAACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Structures

Toxin

• AlphaFold DB: U9XMP3

Antitoxin

• AlphaFold DB: A0A067H947