Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3927315..3927891 Replicon chromosome
Accession NZ_CP069709
Organism Escherichia coli strain EA7

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag JSQ43_RS19480 Protein ID WP_001297643.1
Coordinates 3927604..3927891 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag JSQ43_RS19475 Protein ID WP_000063156.1
Coordinates 3927315..3927617 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ43_RS19460 3923911..3926061 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
JSQ43_RS19465 3926111..3926314 + 204 WP_000467859.1 YbdD/YjiX family protein -
JSQ43_RS19470 3926325..3927281 + 957 WP_001297640.1 GTPase -
JSQ43_RS19475 3927315..3927617 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
JSQ43_RS19480 3927604..3927891 - 288 WP_001297643.1 BrnT family toxin Toxin
JSQ43_RS19485 3928090..3928787 - 698 Protein_3717 IS1 family transposase -
JSQ43_RS19490 3928844..3928939 + 96 Protein_3718 dienelactone hydrolase family protein -
JSQ43_RS19495 3929085..3930317 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
JSQ43_RS19500 3930358..3931638 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3928090..3928299 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T191718 WP_001297643.1 NZ_CP069709:c3927891-3927604 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T191718 NZ_CP091889:c3059360-3059019 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACGCGATATACGTTGCCCGATGACATAGAAACCCATGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT191718 WP_000063156.1 NZ_CP069709:c3927617-3927315 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT191718 NZ_CP091889:c3059590-3059360 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References