Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3817333..3817883 Replicon chromosome
Accession NZ_CP069709
Organism Escherichia coli strain EA7

Toxin (Protein)


Gene name ccdB Uniprot ID D7Y6T6
Locus tag JSQ43_RS18940 Protein ID WP_001160971.1
Coordinates 3817333..3817647 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag JSQ43_RS18945 Protein ID WP_000125566.1
Coordinates 3817650..3817883 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ43_RS18915 3813008..3814294 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
JSQ43_RS18920 3814294..3815283 + 990 WP_000241253.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
JSQ43_RS18925 3815280..3816101 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
JSQ43_RS18930 3816104..3816481 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
JSQ43_RS18935 3816488..3817336 + 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
JSQ43_RS18940 3817333..3817647 - 315 WP_001160971.1 CcdB family protein Toxin
JSQ43_RS18945 3817650..3817883 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
JSQ43_RS18950 3817969..3818448 - 480 WP_001487721.1 type 3 dihydrofolate reductase -
JSQ43_RS18955 3818640..3820502 - 1863 WP_072662688.1 glutathione-regulated potassium-efflux system protein KefC -
JSQ43_RS18960 3820495..3821025 - 531 WP_000600735.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
JSQ43_RS18965 3821132..3822463 - 1332 WP_001183198.1 MFS transporter -
JSQ43_RS18970 3822522..3822809 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.75 Da        Isoelectric Point: 9.0851

>T191717 WP_001160971.1 NZ_CP069709:c3817647-3817333 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T191717 NZ_CP091889:c2987069-2986926 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACGGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT191717 WP_000125566.1 NZ_CP069709:c3817883-3817650 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT191717 NZ_CP091889:2986807-2986993 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCGTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCGATT
GTATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGTG
CAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QVQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References