Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2211675..2212201 Replicon chromosome
Accession NZ_CP069709
Organism Escherichia coli strain EA7

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag JSQ43_RS11110 Protein ID WP_000323025.1
Coordinates 2211914..2212201 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag JSQ43_RS11105 Protein ID WP_000534858.1
Coordinates 2211675..2211914 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ43_RS11075 2207011..2207367 + 357 WP_001315552.1 class I SAM-dependent methyltransferase -
JSQ43_RS11080 2207630..2207812 + 183 Protein_2080 hypothetical protein -
JSQ43_RS11085 2207978..2208264 + 287 Protein_2081 hypothetical protein -
JSQ43_RS11090 2209033..2210373 + 1341 WP_105075516.1 ISNCY family transposase -
JSQ43_RS11095 2210810..2211142 - 333 WP_001301033.1 protein FlxA -
JSQ43_RS11100 2211345..2211650 - 306 WP_071593935.1 hypothetical protein -
JSQ43_RS11105 2211675..2211914 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
JSQ43_RS11110 2211914..2212201 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
JSQ43_RS11115 2212273..2212428 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
JSQ43_RS11120 2212645..2212896 + 252 WP_001463433.1 hypothetical protein -
JSQ43_RS11125 2212963..2213241 + 279 WP_001515373.1 hypothetical protein -
JSQ43_RS11130 2213243..2214292 + 1050 WP_001515372.1 DUF968 domain-containing protein -
JSQ43_RS11135 2214310..2214687 + 378 WP_001204787.1 antitermination protein -
JSQ43_RS11140 2214843..2215367 - 525 WP_105907874.1 outer membrane lipoprotein Blc -
JSQ43_RS11145 2215560..2216519 + 960 WP_105075652.1 DUF523 and DUF1722 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2186779..2265395 78616


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T191712 WP_000323025.1 NZ_CP069709:2211914-2212201 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T191712 NZ_CP091889:613931-614047 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGTTGGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT191712 WP_000534858.1 NZ_CP069709:2211675-2211914 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT191712 NZ_CP091889:c614163-613958 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAGTAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References