Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4273157..4273654 Replicon chromosome
Accession NZ_CP069706
Organism Escherichia coli strain ECY44

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JR341_RS23670 Protein ID WP_001173343.1
Coordinates 4273157..4273330 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JR341_RS23675 Protein ID WP_000398619.1
Coordinates 4273358..4273654 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR341_RS23660 4269827..4271284 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
JR341_RS23665 4271521..4273038 + 1518 WP_001295074.1 lysine--tRNA ligase -
JR341_RS23670 4273157..4273330 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JR341_RS23675 4273358..4273654 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JR341_RS23680 4273881..4274153 - 273 WP_000405647.1 N-acetyltransferase -
JR341_RS23685 4274165..4274395 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
JR341_RS23690 4274576..4276207 + 1632 WP_001216486.1 sensor histidine kinase -
JR341_RS23695 4276204..4276923 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T191677 WP_001173343.1 NZ_CP069706:c4273330-4273157 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T191677 NZ_CP091878:2127830-2127933 [Salmonella enterica subsp. enterica serovar Tennessee]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT191677 WP_000398619.1 NZ_CP069706:c4273654-4273358 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT191677 NZ_CP091878:c2127935-2127792 [Salmonella enterica subsp. enterica serovar Tennessee]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References