Detailed information of TA system TA191672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3500890..3501727
• Replicon: chromosome
• Accession: NZ_CP069706
• Organism: Escherichia coli strain ECY44

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: JR341_RS20000
• Protein ID: WP_000227784.1
• Coordinates: 3501185..3501727 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: JR341_RS19995
• Protein ID: WP_001297137.1
• Coordinates: 3500890..3501201 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR341_RS19970	3495910..3496857	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
JR341_RS19975	3496879..3498870	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
JR341_RS19980	3498860..3499474	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
JR341_RS19985	3499474..3499803	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
JR341_RS19990	3499815..3500705	+	891	WP_000971336.1	heme o synthase	-
JR341_RS19995	3500890..3501201	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
JR341_RS20000	3501185..3501727	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
JR341_RS20005	3501783..3502718	-	936	WP_001368479.1	sel1 repeat family protein	-
JR341_RS20010	3503126..3504490	+	1365	WP_001000960.1	MFS transporter	-
JR341_RS20015	3504618..3505109	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
JR341_RS20020	3505277..3506188	+	912	WP_000705853.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T191672 WP_000227784.1 NZ_CP069706:3501185-3501727 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T191672 NZ_CP091876:1962464-1962652 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT191672 WP_001297137.1 NZ_CP069706:3500890-3501201 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT191672 NZ_CP091876:1962175-1962435 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3