Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3466813..3467431 Replicon chromosome
Accession NZ_CP069706
Organism Escherichia coli strain ECY44

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JR341_RS19830 Protein ID WP_001291435.1
Coordinates 3467213..3467431 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JR341_RS19825 Protein ID WP_000344800.1
Coordinates 3466813..3467187 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR341_RS19815 3461902..3463095 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JR341_RS19820 3463118..3466267 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
JR341_RS19825 3466813..3467187 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JR341_RS19830 3467213..3467431 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JR341_RS19835 3467603..3468154 + 552 WP_000102564.1 maltose O-acetyltransferase -
JR341_RS19840 3468270..3468740 + 471 WP_000136192.1 YlaC family protein -
JR341_RS19845 3468904..3470454 + 1551 WP_001364591.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JR341_RS19850 3470496..3470849 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JR341_RS19860 3471228..3471539 + 312 WP_000409911.1 MGMT family protein -
JR341_RS19865 3471570..3472142 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T191671 WP_001291435.1 NZ_CP069706:3467213-3467431 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T191671 NZ_CP091876:c1945324-1945004 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTTTATTTAAAAGAGTTCGATGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGATGTAAGTTTGGCGACAGAATCAGATGACGCAAAGAAATTAAAAATTACAGAGTA
A

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT191671 WP_000344800.1 NZ_CP069706:3466813-3467187 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT191671 NZ_CP091876:c1945536-1945327 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
TGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References