Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 261860..262491 Replicon chromosome
Accession NZ_CP069706
Organism Escherichia coli strain ECY44

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag JR341_RS04055 Protein ID WP_001259388.1
Coordinates 261860..262135 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JR341_RS04060 Protein ID WP_000593555.1
Coordinates 262132..262491 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR341_RS04040 256864..257931 + 1068 WP_000361467.1 HlyD family secretion protein -
JR341_RS04045 257928..260663 + 2736 WP_000149167.1 ribosome-associated ATPase/putative transporter RbbA -
JR341_RS04050 260663..261787 + 1125 WP_001314210.1 ABC transporter permease -
JR341_RS04055 261860..262135 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
JR341_RS04060 262132..262491 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JR341_RS04065 262611..263012 - 402 WP_001190064.1 nickel-responsive transcriptional regulator NikR -
JR341_RS04070 263018..263824 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
JR341_RS04075 263821..264585 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
JR341_RS04080 264585..265418 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
JR341_RS04085 265415..266359 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T191655 WP_001259388.1 NZ_CP069706:261860-262135 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T191655 NZ_CP091875:c2173140-2172964 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT191655 WP_000593555.1 NZ_CP069706:262132-262491 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT191655 NZ_CP091875:2172841-2172896 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References