Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 42578..43179 Replicon plasmid pECY44-3
Accession NZ_CP069704
Organism Escherichia coli strain ECY44

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag JR341_RS02210 Protein ID WP_001216045.1
Coordinates 42578..42958 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag JR341_RS02215 Protein ID WP_001190712.1
Coordinates 42958..43179 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR341_RS02185 38018..39502 - 1485 WP_000124150.1 terminase -
JR341_RS02190 39502..40695 - 1194 WP_000219607.1 hypothetical protein -
JR341_RS02195 40782..41234 - 453 WP_016236875.1 hypothetical protein -
JR341_RS02200 41323..42366 - 1044 WP_000648821.1 DUF968 domain-containing protein -
JR341_RS02205 42394..42573 - 180 WP_000113018.1 hypothetical protein -
JR341_RS02210 42578..42958 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
JR341_RS02215 42958..43179 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JR341_RS02220 43362..44918 + 1557 WP_016236874.1 type I restriction-modification system subunit M -
JR341_RS02225 45093..45797 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
JR341_RS02230 45865..47163 + 1299 Protein_49 Tn3-like element Tn3 family transposase -
JR341_RS02235 47196..47336 + 141 Protein_50 recombination enhancement function domain protein -
JR341_RS02240 47526..47771 + 246 Protein_51 maturation control protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(3'')-Ia / lnu(F) / blaTEM-1B - 1..105203 105203


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T191652 WP_001216045.1 NZ_CP069704:c42958-42578 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T191652 NZ_CP091875:1971340-1971435 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT191652 WP_001190712.1 NZ_CP069704:c43179-42958 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT191652 NZ_CP091875:c1971520-1971463 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References