Detailed information of TA system TA191645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 9260..9725
• Replicon: plasmid pMUB-MIN6-MCR
• Accession: NZ_CP069695
• Organism: Escherichia coli H20 strain MIN6

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9ZUY2
• Locus tag: JSU10_RS24900
• Protein ID: WP_001230707.1
• Coordinates: 9474..9725 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: JSU10_RS24895
• Protein ID: WP_000801440.1
• Coordinates: 9260..9487 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU10_RS24855 (JSU10_24850)	4490..4705	-	216	WP_000760375.1	EexN family lipoprotein	-
JSU10_RS24860 (JSU10_24855)	4702..4995	-	294	WP_060598542.1	sigma factor-like helix-turn-helix DNA-binding protein	-
JSU10_RS24865 (JSU10_24860)	5072..6301	-	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
JSU10_RS24870 (JSU10_24865)	6305..6808	-	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
JSU10_RS24875 (JSU10_24870)	7169..7516	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	-
JSU10_RS24880 (JSU10_24875)	7513..7761	-	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	-
JSU10_RS24885 (JSU10_24880)	8066..8416	-	351	WP_000854259.1	hypothetical protein	-
JSU10_RS24890 (JSU10_24885)	8507..8806	-	300	WP_000650309.1	hypothetical protein	-
JSU10_RS24895 (JSU10_24890)	9260..9487	-	228	WP_000801440.1	hypothetical protein	Antitoxin
JSU10_RS24900 (JSU10_24895)	9474..9725	-	252	WP_001230707.1	hypothetical protein	Toxin
JSU10_RS24905 (JSU10_24900)	9722..10264	-	543	WP_001083906.1	hypothetical protein	-
JSU10_RS24910 (JSU10_24905)	10369..10884	-	516	WP_001025395.1	J domain-containing protein	-
JSU10_RS24915 (JSU10_24910)	12294..13157	+	864	WP_208235563.1	replication initiation protein	-
JSU10_RS24920 (JSU10_24915)	13170..13574	+	405	WP_012421203.1	hypothetical protein	-
JSU10_RS24925 (JSU10_24920)	13709..14236	+	528	WP_000577043.1	DUF2726 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33288	33288

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9751.13 Da
• Isoelectric Point: 8.9187

>T191645 WP_001230707.1 NZ_CP069695:c9725-9474 [Escherichia coli H20]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T191645 NZ_CP091872:518660-519010 [Bacillus sp. Man122]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT191645 WP_000801440.1 NZ_CP069695:c9487-9260 [Escherichia coli H20]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT191645 NZ_CP091872:518374-518655 [Bacillus sp. Man122]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: U9ZUY2

Antitoxin

• AlphaFold DB: A0A5Y6U8W9