Detailed information of TA system TA191644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 7169..7761
• Replicon: plasmid pMUB-MIN6-MCR
• Accession: NZ_CP069695
• Organism: Escherichia coli H20 strain MIN6

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: JSU10_RS24880
• Protein ID: WP_000520549.1
• Coordinates: 7513..7761 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: JSU10_RS24875
• Protein ID: WP_000681613.1
• Coordinates: 7169..7516 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU10_RS24845 (JSU10_24840)	3175..3501	-	327	WP_000008647.1	TrbC/VirB2 family protein	-
JSU10_RS24850 (JSU10_24845)	3562..4134	-	573	WP_227537772.1	lytic transglycosylase domain-containing protein	-
JSU10_RS24855 (JSU10_24850)	4490..4705	-	216	WP_000760375.1	EexN family lipoprotein	-
JSU10_RS24860 (JSU10_24855)	4702..4995	-	294	WP_060598542.1	sigma factor-like helix-turn-helix DNA-binding protein	-
JSU10_RS24865 (JSU10_24860)	5072..6301	-	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
JSU10_RS24870 (JSU10_24865)	6305..6808	-	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
JSU10_RS24875 (JSU10_24870)	7169..7516	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JSU10_RS24880 (JSU10_24875)	7513..7761	-	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
JSU10_RS24885 (JSU10_24880)	8066..8416	-	351	WP_000854259.1	hypothetical protein	-
JSU10_RS24890 (JSU10_24885)	8507..8806	-	300	WP_000650309.1	hypothetical protein	-
JSU10_RS24895 (JSU10_24890)	9260..9487	-	228	WP_000801440.1	hypothetical protein	-
JSU10_RS24900 (JSU10_24895)	9474..9725	-	252	WP_001230707.1	hypothetical protein	-
JSU10_RS24905 (JSU10_24900)	9722..10264	-	543	WP_001083906.1	hypothetical protein	-
JSU10_RS24910 (JSU10_24905)	10369..10884	-	516	WP_001025395.1	J domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33288	33288

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T191644 WP_000520549.1 NZ_CP069695:c7761-7513 [Escherichia coli H20]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T191644 NZ_CP091864:c232823-232572 [Corynebacterium pseudodiphtheriticum]
ATGAAAACCATGTCTTATTCCGACTCACGAGCACACTATGCAGAGATGCTTGATACCGTGACTGATGACCGTGAAGAAGT
CATCATTACACGCGCAGGACACGAGCCGGTAGTGGTCGTCTCCCTTGATGATTATGAATCATTAAAAGAAACCGCATACC
TACTCAAAAGCCCGGCTAATGCTCGCCGACTACTAGCCTCAATTGAACAGCTCGAAAATGGCGAAGGCACTCAACGGGAT
TTACTCGAATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT191644 WP_000681613.1 NZ_CP069695:c7516-7169 [Escherichia coli H20]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT191644 NZ_CP091864:c232575-232315 [Corynebacterium pseudodiphtheriticum]
ATGAAACTAGTCTGGACTGAATCAGCCTGGGAGGATTACACCTGGTGGCAGACTGAAGACCGGAAGGTGCTCAAACGCAT
CAACAGATTAATCAAAGAGATTCAACGAAATGGAAACTCCGGTATCGGCAAACCAGAACCACTCAAACACGGATTTCAGG
GCTATTGGTCTCGGAGAATCACAGATGAGCACCGGCTAGTTTATAAAATTTTCGGCGATGAAGTTCGCATTGCGTCCTGC
AGATACCACTATGGGCGTTAA

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1