Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 65872..66515 Replicon plasmid pMUB-MIN6-1
Accession NZ_CP069693
Organism Escherichia coli H20 strain MIN6

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag JSU10_RS24370 Protein ID WP_001034046.1
Coordinates 66099..66515 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag JSU10_RS24365 Protein ID WP_001261278.1
Coordinates 65872..66102 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU10_RS24340 (61060) 61060..62595 - 1536 WP_023181050.1 IS21 family transposase -
JSU10_RS24345 (62828) 62828..63178 - 351 Protein_86 RepB family plasmid replication initiator protein -
JSU10_RS24350 (63952) 63952..64758 - 807 WP_000016982.1 site-specific integrase -
JSU10_RS24355 (64759) 64759..65064 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
JSU10_RS24360 (65066) 65066..65284 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
JSU10_RS24365 (65872) 65872..66102 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JSU10_RS24370 (66099) 66099..66515 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
JSU10_RS24375 (66560) 66560..69190 - 2631 WP_251362408.1 hypothetical protein -
JSU10_RS25515 (69187) 69187..69771 - 585 WP_251361774.1 hypothetical protein -
JSU10_RS24385 (70603) 70603..71130 - 528 WP_251362409.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / blaTEM-1B / dfrA1 / ant(3'')-Ia / qacE / sul1 / aph(6)-Id / aph(3'')-Ib / sul2 / tet(B) / sitABCD iutA / iucD / iucC / iucB / iucA 1..89956 89956


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T191643 WP_001034046.1 NZ_CP069693:66099-66515 [Escherichia coli H20]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T191643 NZ_CP091864:c232575-232315 [Corynebacterium pseudodiphtheriticum]
ATGAAACTAGTCTGGACTGAATCAGCCTGGGAGGATTACACCTGGTGGCAGACTGAAGACCGGAAGGTGCTCAAACGCAT
CAACAGATTAATCAAAGAGATTCAACGAAATGGAAACTCCGGTATCGGCAAACCAGAACCACTCAAACACGGATTTCAGG
GCTATTGGTCTCGGAGAATCACAGATGAGCACCGGCTAGTTTATAAAATTTTCGGCGATGAAGTTCGCATTGCGTCCTGC
AGATACCACTATGGGCGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT191643 WP_001261278.1 NZ_CP069693:65872-66102 [Escherichia coli H20]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT191643 NZ_CP091864:c232823-232572 [Corynebacterium pseudodiphtheriticum]
ATGAAAACCATGTCTTATTCCGACTCACGAGCACACTATGCAGAGATGCTTGATACCGTGACTGATGACCGTGAAGAAGT
CATCATTACACGCGCAGGACACGAGCCGGTAGTGGTCGTCTCCCTTGATGATTATGAATCATTAAAAGAAACCGCATACC
TACTCAAAAGCCCGGCTAATGCTCGCCGACTACTAGCCTCAATTGAACAGCTCGAAAATGGCGAAGGCACTCAACGGGAT
TTACTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References