Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 64759..65284 | Replicon | plasmid pMUB-MIN6-1 |
| Accession | NZ_CP069693 | ||
| Organism | Escherichia coli H20 strain MIN6 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | V0SSI5 |
| Locus tag | JSU10_RS24355 | Protein ID | WP_001159868.1 |
| Coordinates | 64759..65064 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | S1PPD8 |
| Locus tag | JSU10_RS24360 | Protein ID | WP_000813634.1 |
| Coordinates | 65066..65284 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JSU10_RS24330 (59778) | 59778..60188 | - | 411 | Protein_83 | RepB family plasmid replication initiator protein | - |
| JSU10_RS24335 (60288) | 60288..61043 | - | 756 | WP_001282653.1 | IS21-like element ISSso4 family helper ATPase IstB | - |
| JSU10_RS24340 (61060) | 61060..62595 | - | 1536 | WP_023181050.1 | IS21 family transposase | - |
| JSU10_RS24345 (62828) | 62828..63178 | - | 351 | Protein_86 | RepB family plasmid replication initiator protein | - |
| JSU10_RS24350 (63952) | 63952..64758 | - | 807 | WP_000016982.1 | site-specific integrase | - |
| JSU10_RS24355 (64759) | 64759..65064 | - | 306 | WP_001159868.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| JSU10_RS24360 (65066) | 65066..65284 | - | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| JSU10_RS24365 (65872) | 65872..66102 | + | 231 | WP_001261278.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| JSU10_RS24370 (66099) | 66099..66515 | + | 417 | WP_001034046.1 | type II toxin-antitoxin system VapC family toxin | - |
| JSU10_RS24375 (66560) | 66560..69190 | - | 2631 | WP_251362408.1 | hypothetical protein | - |
| JSU10_RS25515 (69187) | 69187..69771 | - | 585 | WP_251361774.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aph(3')-Ia / blaTEM-1B / dfrA1 / ant(3'')-Ia / qacE / sul1 / aph(6)-Id / aph(3'')-Ib / sul2 / tet(B) / sitABCD | iutA / iucD / iucC / iucB / iucA | 1..89956 | 89956 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11706.51 Da Isoelectric Point: 6.4674
>T191642 WP_001159868.1 NZ_CP069693:c65064-64759 [Escherichia coli H20]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T191642 NZ_CP091861:c4883200-4882907 [Flavobacterium bizetiae]
ATGGCTAAATATTATTTAACCAATAAAGCAGTTGAAGATTTGACTAATATTTGGGATTATACTTATGATGAATGGTCAGA
AAATCAGGCAGATAAATATTATAATTTACTTTTGAGTTCTTGCCAGGAAATTGCCGAAAATCCCAATCTCGGCAAAAAAT
ATGATAATATAACAGAGAAATTATTAGGTTTTAAATCGAATCAGCATATAATTTTTTATCAGATTATTTCAAACACTGAA
GTTGAAATTATTAGAATTCTTCATGGAAGAATGGATTTGAAAAGTAAATTTTAA
ATGGCTAAATATTATTTAACCAATAAAGCAGTTGAAGATTTGACTAATATTTGGGATTATACTTATGATGAATGGTCAGA
AAATCAGGCAGATAAATATTATAATTTACTTTTGAGTTCTTGCCAGGAAATTGCCGAAAATCCCAATCTCGGCAAAAAAT
ATGATAATATAACAGAGAAATTATTAGGTTTTAAATCGAATCAGCATATAATTTTTTATCAGATTATTTCAAACACTGAA
GTTGAAATTATTAGAATTCTTCATGGAAGAATGGATTTGAAAAGTAAATTTTAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8372.34 Da Isoelectric Point: 4.7405
>AT191642 WP_000813634.1 NZ_CP069693:c65284-65066 [Escherichia coli H20]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT191642 NZ_CP091861:c4883441-4883193 [Flavobacterium bizetiae]
ATGGGACGAAATACTTCAATATCGCTGGGAAATCATTTTGAAAGCTTCATAGAAAATAGTCTTTCTGAAGGAAGATTTAA
AAACGCTAGTGAAGTGGTTAGAGCCGGATTACGACTTTTGGAAGAAGAAGAAAATAAATTAATTATTTTAAAAAAAGCCG
TTCAGGACGGAATTGATAGCGGACGTGCTGAAAATTTCGATGCTAAGAAACATCTTGAAAGCTTAAAAGCAAAGAAAAGA
AATGGCTAA
ATGGGACGAAATACTTCAATATCGCTGGGAAATCATTTTGAAAGCTTCATAGAAAATAGTCTTTCTGAAGGAAGATTTAA
AAACGCTAGTGAAGTGGTTAGAGCCGGATTACGACTTTTGGAAGAAGAAGAAAATAAATTAATTATTTTAAAAAAAGCCG
TTCAGGACGGAATTGATAGCGGACGTGCTGAAAATTTCGATGCTAAGAAACATCTTGAAAGCTTAAAAGCAAAGAAAAGA
AATGGCTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|