Detailed information of TA system TA191636

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 4489984..4490481
• Replicon: chromosome
• Accession: NZ_CP069692
• Organism: Escherichia coli H20 strain MIN6

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: JSU10_RS21650
• Protein ID: WP_001173343.1
• Coordinates: 4489984..4490157 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: JSU10_RS21655
• Protein ID: WP_071988580.1
• Coordinates: 4490185..4490481 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU10_RS21640 (4486654)	4486654..4488111	+	1458	WP_000856829.1	dipeptide/tripeptide permease DtpC	-
JSU10_RS21645 (4488348)	4488348..4489865	+	1518	WP_001295087.1	lysine--tRNA ligase	-
JSU10_RS21650 (4489984)	4489984..4490157	-	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
JSU10_RS21655 (4490185)	4490185..4490481	-	297	WP_071988580.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
JSU10_RS21660 (4490708)	4490708..4490980	-	273	WP_000405647.1	GNAT family N-acetyltransferase	-
JSU10_RS21665 (4491193)	4491193..4495122	-	3930	WP_046201569.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	espX5 / espX4 / espX4	4445817..4634211	188394

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T191636 WP_001173343.1 NZ_CP069692:c4490157-4489984 [Escherichia coli H20]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T191636 NZ_CP091847:17449-17733 [Klebsiella pneumoniae]
ATGACCTATAAGTTAGCCTTCAATGAATCAGCTTTGAAAGAATGGAAAAAACTGGGTCATACTATTCGGGAGCAATTCAA
AAAGAAGCTTTCCGAACGGCTTCAGAACCCACGGGTGCCCGCAGCACAACTCCATGGAGGTAAAGACCAGTATAAAATAA
AGCTCCATGGCGCGGGTTACCGACTGGTTTACAGTGTCAACGATGAAATTGTGACGGTCACCGTAATTGGGGTGGGCAAA
CGCAACAATGATGATATCTACAACGCAACCCGACACAGAAATTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11482.00 Da
• Isoelectric Point: 4.1705

>AT191636 WP_071988580.1 NZ_CP069692:c4490481-4490185 [Escherichia coli H20]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSTIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT191636 NZ_CP091847:17211-17459 [Klebsiella pneumoniae]
ATGGCATTTCAGGTTTTAACCACCACAGCAGCCAGCATTACAGAACTTAAACGTGACCCTATGGGTACCTTCAATGCAGG
AGAAGGTGCACCGGTAGCAATCCTGAATCGCAACGAACCCGCTTTTTACTGTGTACCACCAGCCCTGTATGCTCAGCTGA
TGGAAATGCTGGAAGATCAAGAGTTGGGGCGTATTGCTGATGAACGAATCGGTGAGGACCGAATCGAGGTCAACATTGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: A0A7U9QI41