Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4477146..4477944 Replicon chromosome
Accession NZ_CP069692
Organism Escherichia coli H20 strain MIN6

Toxin (Protein)


Gene name yeeV Uniprot ID U9XMP3
Locus tag JSU10_RS21595 Protein ID WP_000854735.1
Coordinates 4477567..4477944 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A067H947
Locus tag JSU10_RS21590 Protein ID WP_001285415.1
Coordinates 4477146..4477520 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU10_RS21555 (4473077) 4473077..4473757 + 681 WP_001282919.1 WYL domain-containing protein -
JSU10_RS21560 (4473905) 4473905..4474582 + 678 WP_001097302.1 hypothetical protein -
JSU10_RS21565 (4474588) 4474588..4474821 + 234 WP_001278287.1 DUF905 family protein -
JSU10_RS21570 (4474911) 4474911..4475729 + 819 WP_001175148.1 DUF932 domain-containing protein -
JSU10_RS21575 (4475811) 4475811..4476290 + 480 WP_000844100.1 antirestriction protein -
JSU10_RS21580 (4476306) 4476306..4476782 + 477 WP_001366855.1 RadC family protein -
JSU10_RS21585 (4476845) 4476845..4477066 + 222 WP_000692309.1 DUF987 domain-containing protein -
JSU10_RS21590 (4477146) 4477146..4477520 + 375 WP_001285415.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
JSU10_RS21595 (4477567) 4477567..4477944 + 378 WP_000854735.1 TA system toxin CbtA family protein Toxin
JSU10_RS21600 (4477941) 4477941..4478429 + 489 WP_000761676.1 DUF5983 family protein -
JSU10_RS21605 (4478441) 4478441..4478638 + 198 WP_000839291.1 DUF957 domain-containing protein -
JSU10_RS21610 (4478723) 4478723..4479589 + 867 WP_001280433.1 DUF4942 domain-containing protein -
JSU10_RS21615 (4479661) 4479661..4479924 + 264 WP_001143297.1 type II toxin-antitoxin system ParD family antitoxin -
JSU10_RS21620 (4479921) 4479921..4480247 + 327 WP_000779483.1 type II toxin-antitoxin system RelE/ParE family toxin -
JSU10_RS21625 (4481130) 4481130..4482668 + 1539 WP_001187170.1 lysine decarboxylation/transport transcriptional activator CadC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - espX5 / espX4 / espX4 4445817..4634211 188394
- flank IS/Tn - - 4471646..4472626 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14061.07 Da        Isoelectric Point: 8.2904

>T191635 WP_000854735.1 NZ_CP069692:4477567-4477944 [Escherichia coli H20]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T191635 NZ_CP091846:c5226224-5225841 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13663.32 Da        Isoelectric Point: 5.8640

>AT191635 WP_001285415.1 NZ_CP069692:4477146-4477520 [Escherichia coli H20]
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT191635 NZ_CP091846:c5226466-5226224 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XMP3

Antitoxin

Source ID Structure
AlphaFold DB A0A067H947

References