Detailed information of TA system TA191633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 4262973..4264308
• Replicon: chromosome
• Accession: NZ_CP069692
• Organism: Escherichia coli H20 strain MIN6

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: F4VMR9
• Locus tag: JSU10_RS20535
• Protein ID: WP_000342497.1
• Coordinates: 4262973..4263920 (-)
• Length: 316 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: A0A228Z6F8
• Locus tag: JSU10_RS20540
• Protein ID: WP_000152739.1
• Coordinates: 4263913..4264308 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU10_RS20510 (4258826)	4258826..4259542	+	717	WP_001295734.1	N-acetylneuraminic acid outer membrane channel NanC	-
JSU10_RS20515 (4259562)	4259562..4260668	+	1107	WP_001044128.1	N-acetylneuraminate epimerase	-
JSU10_RS20520 (4260733)	4260733..4261713	+	981	WP_001366032.1	sialate O-acetylesterase	-
JSU10_RS20525 (4261721)	4261721..4262371	-	651	WP_001037970.1	HNH endonuclease	-
JSU10_RS25465 (4262508)	4262508..4262651	+	144	Protein_4072	HNH endonuclease	-
JSU10_RS20530 (4262750)	4262750..4262935	+	186	WP_000066585.1	hypothetical protein	-
JSU10_RS20535 (4262973)	4262973..4263920	-	948	WP_000342497.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
JSU10_RS20540 (4263913)	4263913..4264308	-	396	WP_000152739.1	DUF6088 family protein	Antitoxin
JSU10_RS20545 (4264378)	4264378..4265211	-	834	WP_001192741.1	DUF4942 domain-containing protein	-
JSU10_RS20550 (4265517)	4265517..4267073	+	1557	WP_000202816.1	type I restriction-modification system subunit M	-
JSU10_RS20555 (4267070)	4267070..4268236	+	1167	WP_001293040.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	4256768..4268236	11468

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-303)

Antitoxin

• Protein of unknown function DUF6088 (7-108)

Sequences

Toxin

Protein

• Length: 316 a.a.
• Molecular weight: 35588.79 Da
• Isoelectric Point: 4.9737

>T191633 WP_000342497.1 NZ_CP069692:c4263920-4262973 [Escherichia coli H20]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA

Nucleotide

• Length: 948 bp

>T191633 NZ_CP091846:c4748513-4748229 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14605.90 Da
• Isoelectric Point: 11.6915

>AT191633 WP_000152739.1 NZ_CP069692:c4264308-4263913 [Escherichia coli H20]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRDLVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Nucleotide

• Length: 396 bp

>AT191633 NZ_CP091846:c4748745-4748503 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A1L5BAQ6

Antitoxin

• AlphaFold DB: A0A228Z6F8