Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3650567..3651185 Replicon chromosome
Accession NZ_CP069692
Organism Escherichia coli H20 strain MIN6

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JSU10_RS17655 Protein ID WP_001291435.1
Coordinates 3650967..3651185 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JSU10_RS17650 Protein ID WP_000344800.1
Coordinates 3650567..3650941 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU10_RS17640 (3645656) 3645656..3646849 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JSU10_RS17645 (3646872) 3646872..3650021 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JSU10_RS17650 (3650567) 3650567..3650941 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JSU10_RS17655 (3650967) 3650967..3651185 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JSU10_RS17660 (3651356) 3651356..3651907 + 552 WP_000102556.1 maltose O-acetyltransferase -
JSU10_RS17665 (3652023) 3652023..3652493 + 471 WP_000136192.1 YlaC family protein -
JSU10_RS17670 (3652657) 3652657..3654207 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JSU10_RS17675 (3654249) 3654249..3654602 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JSU10_RS17685 (3654981) 3654981..3655292 + 312 WP_000409911.1 MGMT family protein -
JSU10_RS17690 (3655323) 3655323..3655895 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T191631 WP_001291435.1 NZ_CP069692:3650967-3651185 [Escherichia coli H20]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T191631 NZ_CP091846:4031343-4031561 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT191631 WP_000344800.1 NZ_CP069692:3650567-3650941 [Escherichia coli H20]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT191631 NZ_CP091846:4030942-4031316 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References