Detailed information of TA system TA191627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2575871..2576509
• Replicon: chromosome
• Accession: NZ_CP069692
• Organism: Escherichia coli H20 strain MIN6

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JSU10_RS12330
• Protein ID: WP_000813794.1
• Coordinates: 2576333..2576509 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JSU10_RS12325
• Protein ID: WP_001270286.1
• Coordinates: 2575871..2576287 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU10_RS12305 (2571023)	2571023..2571964	-	942	WP_001251313.1	ABC transporter permease	-
JSU10_RS12310 (2571965)	2571965..2572978	-	1014	WP_001542893.1	ABC transporter ATP-binding protein	-
JSU10_RS12315 (2572996)	2572996..2574141	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
JSU10_RS12320 (2574386)	2574386..2575792	-	1407	WP_000760663.1	PLP-dependent aminotransferase family protein	-
JSU10_RS12325 (2575871)	2575871..2576287	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JSU10_RS12330 (2576333)	2576333..2576509	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JSU10_RS12335 (2576731)	2576731..2576961	+	231	WP_000494244.1	YncJ family protein	-
JSU10_RS12340 (2577053)	2577053..2579014	-	1962	WP_001442195.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JSU10_RS12345 (2579087)	2579087..2579623	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
JSU10_RS12350 (2579715)	2579715..2580890	+	1176	WP_001583501.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2580930..2582078	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T191627 WP_000813794.1 NZ_CP069692:c2576509-2576333 [Escherichia coli H20]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T191627 NZ_CP091846:351517-351864 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT191627 WP_001270286.1 NZ_CP069692:c2576287-2575871 [Escherichia coli H20]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT191627 NZ_CP091846:351864-352163 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• PDB: 6HPB