191616

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1439222..1439847 Replicon chromosome
Accession NZ_CP069692
Organism Escherichia coli H20 strain MIN6

Toxin (Protein)


Gene name vapC Uniprot ID A0A2J1DF47
Locus tag JSU10_RS07005 Protein ID WP_000911331.1
Coordinates 1439449..1439847 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag JSU10_RS07000 Protein ID WP_000450524.1
Coordinates 1439222..1439449 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU10_RS06975 (1435026) 1435026..1435496 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
JSU10_RS06980 (1435496) 1435496..1436068 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
JSU10_RS06985 (1436214) 1436214..1437092 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
JSU10_RS06990 (1437109) 1437109..1438143 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
JSU10_RS06995 (1438355) 1438355..1439068 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
JSU10_RS07000 (1439222) 1439222..1439449 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
JSU10_RS07005 (1439449) 1439449..1439847 + 399 WP_000911331.1 type II toxin-antitoxin system VapC family toxin Toxin
JSU10_RS07010 (1439994) 1439994..1440857 + 864 WP_001267507.1 neutral zinc metallopeptidase -
JSU10_RS07015 (1440872) 1440872..1442887 + 2016 WP_001543081.1 tRNA cytosine(34) acetyltransferase TmcA -
JSU10_RS07020 (1442961) 1442961..1443659 + 699 WP_000679812.1 esterase -
JSU10_RS07025 (1443769) 1443769..1443969 - 201 WP_089631931.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14862.19 Da        Isoelectric Point: 9.2216

>T191616 WP_000911331.1 NZ_CP069692:1439449-1439847 [Escherichia coli H20]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLVELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T191616 NZ_CP091839:c2302918-2302604 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT191616 WP_000450524.1 NZ_CP069692:1439222-1439449 [Escherichia coli H20]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT191616 NZ_CP091839:c2302607-2302191 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J1DF47

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References